Detailed information of TA system TA227554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): AbkB-brnA/BrnT-BrnA
• Location: 296770..297358
• Replicon: plasmid pRIVM_C011601_1
• Accession: NZ_CP089039
• Organism: Acinetobacter ursingii strain RIVM_C011601

Toxin (Protein)

• Gene name: AbkB
• Uniprot ID: -
• Locus tag: LSO59_RS18400
• Protein ID: WP_130114512.1
• Coordinates: 296770..297057 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LSO59_RS18405
• Protein ID: WP_130114513.1
• Coordinates: 297044..297358 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSO59_RS18370 (LSO59_18340)	291960..292802	+	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
LSO59_RS18375 (LSO59_18345)	292893..293599	+	707	Protein_282	IS1 family transposase	-
LSO59_RS18380 (LSO59_18350)	293773..294123	+	351	Protein_283	AraC family ligand binding domain-containing protein	-
LSO59_RS18385 (LSO59_18355)	294207..295339	+	1133	WP_223155595.1	IS3-like element ISAba14 family transposase	-
LSO59_RS18390 (LSO59_18360)	295466..295885	+	420	WP_263503917.1	AraC family transcriptional regulator	-
LSO59_RS18395 (LSO59_18365)	295935..296540	+	606	WP_000447788.1	LysE family translocator	-
LSO59_RS18400 (LSO59_18370)	296770..297057	+	288	WP_130114512.1	BrnT family toxin	Toxin
LSO59_RS18405 (LSO59_18375)	297044..297358	+	315	WP_130114513.1	BrnA antitoxin family protein	Antitoxin
LSO59_RS18410 (LSO59_18380)	297435..297662	-	228	Protein_289	ISNCY family transposase	-
LSO59_RS18415 (LSO59_18385)	297714..298418	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
LSO59_RS18420 (LSO59_18390)	298482..299189	+	708	Protein_291	IS3 family transposase	-
LSO59_RS18425 (LSO59_18395)	299381..299746	-	366	WP_130114467.1	copper-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / blaCARB-2 / qacE / sul1 / dfrA19 / blaIMP-4 / catB3 / ARR-3 / msr(E) / mph(E) / aac(3)-IId / blaOXA-58	-	1..317317	317317
	-	inside	IScluster/Tn	blaOXA-58	-	287428..299189	11761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 6.0900

>T227554 WP_130114512.1 NZ_CP089039:296770-297057 [Acinetobacter ursingii]
MKQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T227554 NZ_CP122946:c1078431-1078165 [Helicobacter pylori]
GTGTTGAAACTCAACCTTAAAAAATCTTTTCAAAAAGATTTTGATAAATTACTTTTGAATGGGTTTGATGATAGCGTTTT
GAATAAGGTCATTCTGTCTTTGAGAAAAAAAGAACCGCTAGACCCCCAATTTCAAGATCATGCTTTAAAGGGAAAGTGGA
AACCTTTTAGGGAATGCCACATCAAAGCTGATATTTTGCTTGTGTATTTGGTGAAAGATGATGAGCTTATTTTAGTAAGA
CTAGGCAGTCATAGCGAGCTGTTTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12254.89 Da
• Isoelectric Point: 7.4024

>AT227554 WP_130114513.1 NZ_CP089039:297044-297358 [Acinetobacter ursingii]
MSMVRHSRKELNEKFSDKQDAEIERLLAKGTVPDDHLDLSDMPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT227554 NZ_CP122946:c1078789-1078412 [Helicobacter pylori]
ATGCCTAACACCACCGCCAAAAAAGACTACACACAATACAGCGAAAAACAGCTTTTTAACTTAATCAACCAATTAGAGCA
AAAAATCAAAAAGATGCAAAATGATAGAGCTTCTTTTAAAGAAAAAATGGCTAAAGAATTGGACAAAAGGGATCAAAACT
TTAAGGATAAAATAGACGCATTAAATGGACTCTTGCAAAAAATCAGCCAAGCTTTTGATGATAAAAGAGATTGTTGTTTG
GGGCGCAAGATCCCAAACCTTGAAACGCAACAAGCCATGAGAGATGCGCTTAACAAAGAAACCGATTTGATTGTTGAGGA
TTTTTCTAGTTATAGCGATGAAAGAAAAAGGGCGTTAGGTGTTGAAACTCAACCTTAA