227551

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1771..2330 Replicon plasmid pO157
Accession NZ_CP089037
Organism Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag CO544_RS27340 Protein ID WP_000421248.1
Coordinates 2055..2330 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag CO544_RS27335 Protein ID WP_001178089.1
Coordinates 1771..2055 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO544_RS27330 (1) 1..858 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
CO544_RS27335 (1771) 1771..2055 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
CO544_RS27340 (2055) 2055..2330 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CO544_RS27345 (2425) 2425..2631 + 207 WP_001105064.1 hypothetical protein -
CO544_RS27350 (2731) 2731..2946 - 216 Protein_5 transposase -
CO544_RS27355 (2990) 2990..3556 - 567 Protein_6 IS3-like element IS600 family transposase -
CO544_RS27360 (3556) 3556..3972 - 417 Protein_7 IS91 family transposase -
CO544_RS27365 (3972) 3972..4157 - 186 WP_001302179.1 hypothetical protein -
CO544_RS27370 (4334) 4334..6544 + 2211 WP_000592771.1 catalase/peroxidase KatP -
CO544_RS27375 (6588) 6588..6977 + 390 WP_001172748.1 cytochrome b562 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92418 92418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T227551 WP_000421248.1 NZ_CP089037:2055-2330 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T227551 NZ_CP122940:319-834 [Escherichia coli]
ATGAAATTTTACACAATATCAAGCAGTTACACAAAATACCTGAAGGACTTTGATGACAAAGTTCCCAATAGCGAAGATCC
TACATACAACAATCCTAAGGCTTTCATTGGCATAGTACTAGAGATTGAAGGACATAAATATTTAGCACCTTTAACGTCGC
CAAAAGCATGGCATGCTAATGTAAAAGAGTCATCACCCGCCTTCTTCAAACTTCATGAAAACGGTGTCCCTGACAATCAG
CTTGGATTGATTAATCTGAAATTTATGATTCCAATAATCGAAGCTGAAGTGTCTTTACTTGATCTGGATAGCATGCCTGA
TACGCCTTACAAAAGAATGCTATATAAACAGTTACAATTTATTCGTGTAAATGAAGATAAAATATCTGAAAAATCAAAAC
TATTAAGAAACCTTGCCTTGCAAGGCAGAATGCAAGGAACATGCGACTTTGCCGTATTAGAAGAAAAATATCAACATTTC
GGTAAAAAACCTGAAGACATGGAAATAGACGATTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT227551 WP_001178089.1 NZ_CP089037:1771-2055 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT227551 NZ_CP122940:210-267 [Escherichia coli]
AGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCTTAAAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References