Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5010459..5010956 Replicon chromosome
Accession NZ_CP089036
Organism Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag CO544_RS25175 Protein ID WP_001173343.1
Coordinates 5010459..5010632 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag CO544_RS25180 Protein ID WP_000398619.1
Coordinates 5010660..5010956 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO544_RS25165 (5007129) 5007129..5008586 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
CO544_RS25170 (5008823) 5008823..5010340 + 1518 WP_001295074.1 lysine--tRNA ligase -
CO544_RS25175 (5010459) 5010459..5010632 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
CO544_RS25180 (5010660) 5010660..5010956 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
CO544_RS25185 (5011183) 5011183..5011455 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
CO544_RS25190 (5011467) 5011467..5011697 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
CO544_RS25195 (5011878) 5011878..5013509 + 1632 WP_001216477.1 sensor histidine kinase -
CO544_RS25200 (5013506) 5013506..5014225 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T227550 WP_001173343.1 NZ_CP089036:c5010632-5010459 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T227550 NZ_CP122938:c4879189-4878878 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT227550 WP_000398619.1 NZ_CP089036:c5010956-5010660 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT227550 NZ_CP122938:c4878877-4878587 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References