Detailed information of TA system TA227546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4665767..4666317
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: CO544_RS23630
• Protein ID: WP_231824931.1
• Coordinates: 4665767..4666081 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: CO544_RS23635
• Protein ID: WP_000125566.1
• Coordinates: 4666084..4666317 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO544_RS23605 (4661442)	4661442..4662728	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
CO544_RS23610 (4662728)	4662728..4663717	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
CO544_RS23615 (4663714)	4663714..4664535	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
CO544_RS23620 (4664538)	4664538..4664915	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
CO544_RS23625 (4664922)	4664922..4665770	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
CO544_RS23630 (4665767)	4665767..4666081	-	315	WP_231824931.1	CcdB family protein	Toxin
CO544_RS23635 (4666084)	4666084..4666317	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
CO544_RS23640 (4666403)	4666403..4666882	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
CO544_RS23645 (4667074)	4667074..4668936	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
CO544_RS23650 (4668929)	4668929..4669459	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
CO544_RS23655 (4669567)	4669567..4670898	-	1332	WP_001183177.1	MFS transporter	-
CO544_RS23660 (4670956)	4670956..4671243	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11687.78 Da
• Isoelectric Point: 9.8515

>T227546 WP_231824931.1 NZ_CP089036:c4666081-4665767 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASALRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T227546 NZ_CP122938:c4011046-4010648 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT227546 WP_000125566.1 NZ_CP089036:c4666317-4666084 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT227546 NZ_CP122938:c4011342-4011049 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9