Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4169174..4169792 Replicon chromosome
Accession NZ_CP089036
Organism Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag CO544_RS21305 Protein ID WP_001291435.1
Coordinates 4169574..4169792 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag CO544_RS21300 Protein ID WP_000344800.1
Coordinates 4169174..4169548 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO544_RS21290 (4164263) 4164263..4165456 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CO544_RS21295 (4165479) 4165479..4168628 + 3150 WP_001132452.1 efflux RND transporter permease AcrB -
CO544_RS21300 (4169174) 4169174..4169548 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
CO544_RS21305 (4169574) 4169574..4169792 + 219 WP_001291435.1 HHA domain-containing protein Toxin
CO544_RS21310 (4169964) 4169964..4170515 + 552 WP_000102553.1 maltose O-acetyltransferase -
CO544_RS21315 (4170631) 4170631..4171101 + 471 WP_000136192.1 YlaC family protein -
CO544_RS21320 (4171265) 4171265..4172815 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
CO544_RS21325 (4172857) 4172857..4173210 - 354 WP_000878140.1 DUF1428 domain-containing protein -
CO544_RS21335 (4173589) 4173589..4173900 + 312 WP_000409911.1 MGMT family protein -
CO544_RS21340 (4173933) 4173933..4174505 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227544 WP_001291435.1 NZ_CP089036:4169574-4169792 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T227544 NZ_CP122938:3786236-3786454 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT227544 WP_000344800.1 NZ_CP089036:4169174-4169548 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT227544 NZ_CP122938:3785836-3786210 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References