Detailed information of TA system TA227535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2625264..2625735
• Replicon: chromosome
• Accession: NZ_CP089036
• Organism: Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: CO544_RS13400
• Protein ID: WP_001303511.1
• Coordinates: 2625264..2625542 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: CO544_RS13405
• Protein ID: WP_001302048.1
• Coordinates: 2625544..2625735 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO544_RS13375 (2622199)	2622199..2623284	-	1086	Protein_2625	exonuclease	-
CO544_RS13380 (2623377)	2623377..2623568	-	192	WP_001090196.1	DUF1482 family protein	-
CO544_RS13385 (2623565)	2623565..2623753	-	189	WP_000449192.1	cell division inhibition protein DicB	-
CO544_RS13390 (2624322)	2624322..2624540	-	219	WP_001171930.1	protein YdfC	-
CO544_RS13395 (2624612)	2624612..2624911	-	300	WP_001240334.1	hypothetical protein	-
CO544_RS13400 (2625264)	2625264..2625542	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO544_RS13405 (2625544)	2625544..2625735	-	192	WP_001302048.1	hypothetical protein	Antitoxin
CO544_RS13410 (2625756)	2625756..2626127	-	372	WP_001169686.1	hypothetical protein	-
CO544_RS13415 (2626225)	2626225..2626527	+	303	WP_000172738.1	transcriptional regulator	-
CO544_RS13420 (2626524)	2626524..2626949	+	426	WP_000693943.1	toxin YdaT family protein	-
CO544_RS13425 (2626972)	2626972..2627934	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
CO544_RS13430 (2627941)	2627941..2628681	+	741	WP_000788938.1	ATP-binding protein	-
CO544_RS13435 (2628707)	2628707..2629476	+	770	Protein_2637	DUF1627 domain-containing protein	-
CO544_RS13440 (2629492)	2629492..2629887	+	396	WP_001118159.1	DUF977 family protein	-
CO544_RS13445 (2629944)	2629944..2630528	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2609071..2709914	100843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T227535 WP_001303511.1 NZ_CP089036:c2625542-2625264 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T227535 NZ_CP122938:1893832-1894086 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT227535 WP_001302048.1 NZ_CP089036:c2625735-2625544 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT227535 NZ_CP122938:1893584-1893835 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• AlphaFold DB: A0A829CTP0