227521

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 401824..402624 Replicon chromosome
Accession NZ_CP089036
Organism Escherichia coli O157:H7 strain 7386WT

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag CO544_RS01925 Protein ID WP_000342448.1
Coordinates 402097..402624 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag CO544_RS01920 Protein ID WP_001277108.1
Coordinates 401824..402090 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO544_RS01900 (397482) 397482..398150 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
CO544_RS01905 (398143) 398143..399201 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
CO544_RS01910 (399446) 399446..400300 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
CO544_RS01915 (400571) 400571..401674 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
CO544_RS01920 (401824) 401824..402090 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
CO544_RS01925 (402097) 402097..402624 + 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
CO544_RS01930 (402621) 402621..403004 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
CO544_RS01935 (403428) 403428..404537 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
CO544_RS01940 (404585) 404585..405511 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
CO544_RS01945 (405508) 405508..406785 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
CO544_RS01950 (406782) 406782..407549 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T227521 WP_000342448.1 NZ_CP089036:402097-402624 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T227521 NZ_CP122929:2661744-2661851 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT227521 WP_001277108.1 NZ_CP089036:401824-402090 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT227521 NZ_CP122929:c2661697-2661631 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
AlphaFold DB A0A829CN24

References