Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5195288..5195785 Replicon chromosome
Accession NZ_CP089034
Organism Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LSI70_RS26375 Protein ID WP_001173343.1
Coordinates 5195288..5195461 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LSI70_RS26380 Protein ID WP_000398619.1
Coordinates 5195489..5195785 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI70_RS26365 (5191958) 5191958..5193415 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
LSI70_RS26370 (5193652) 5193652..5195169 + 1518 WP_001295074.1 lysine--tRNA ligase -
LSI70_RS26375 (5195288) 5195288..5195461 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LSI70_RS26380 (5195489) 5195489..5195785 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LSI70_RS26385 (5196012) 5196012..5196284 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LSI70_RS26390 (5196296) 5196296..5196526 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LSI70_RS26395 (5196707) 5196707..5198338 + 1632 WP_001216477.1 sensor histidine kinase -
LSI70_RS26400 (5198335) 5198335..5199054 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T227516 WP_001173343.1 NZ_CP089034:c5195461-5195288 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T227516 NZ_CP122929:2027136-2027239 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT227516 WP_000398619.1 NZ_CP089034:c5195785-5195489 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT227516 NZ_CP122929:c2027277-2027006 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References