Detailed information of TA system TA227515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5098297..5098892
• Replicon: chromosome
• Accession: NZ_CP089034
• Organism: Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: LSI70_RS25875
• Protein ID: WP_000239579.1
• Coordinates: 5098297..5098647 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: LSI70_RS25880
• Protein ID: WP_001223210.1
• Coordinates: 5098641..5098892 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI70_RS25855 (5093743)	5093743..5094765	-	1023	WP_001301928.1	ABC transporter permease	-
LSI70_RS25860 (5094779)	5094779..5096281	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
LSI70_RS25865 (5096421)	5096421..5097377	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LSI70_RS25870 (5097687)	5097687..5098217	+	531	WP_000055075.1	inorganic diphosphatase	-
LSI70_RS25875 (5098297)	5098297..5098647	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
LSI70_RS25880 (5098641)	5098641..5098892	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LSI70_RS25885 (5099104)	5099104..5099445	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LSI70_RS25890 (5099448)	5099448..5103227	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T227515 WP_000239579.1 NZ_CP089034:c5098647-5098297 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T227515 NZ_CP122929:c1840797-1840423 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT227515 WP_001223210.1 NZ_CP089034:c5098892-5098641 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT227515 NZ_CP122929:c1841254-1840886 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8