Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3380518..3381313 Replicon chromosome
Accession NZ_CP089034
Organism Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag LSI70_RS17505 Protein ID WP_000854914.1
Coordinates 3380518..3380892 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0B0VS41
Locus tag LSI70_RS17510 Protein ID WP_001280954.1
Coordinates 3380939..3381313 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI70_RS17465 (3375522) 3375522..3376013 - 492 WP_001301587.1 DUF1097 domain-containing protein -
LSI70_RS17470 (3376115) 3376115..3376669 - 555 WP_001001917.1 molecular chaperone YcdY -
LSI70_RS17475 (3376693) 3376693..3377430 - 738 WP_000283664.1 zinc-binding phosphatase -
LSI70_RS17480 (3377485) 3377485..3378423 - 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
LSI70_RS17490 (3378894) 3378894..3379736 - 843 WP_001280481.1 DUF4942 domain-containing protein -
LSI70_RS17495 (3379821) 3379821..3380018 - 198 WP_000839282.1 DUF957 domain-containing protein -
LSI70_RS17500 (3380030) 3380030..3380521 - 492 WP_000976853.1 DUF5983 family protein -
LSI70_RS17505 (3380518) 3380518..3380892 - 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
LSI70_RS17510 (3380939) 3380939..3381313 - 375 WP_001280954.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
LSI70_RS17515 (3381476) 3381476..3381697 - 222 WP_001220314.1 DUF987 domain-containing protein -
LSI70_RS17520 (3381760) 3381760..3382236 - 477 WP_001186738.1 RadC family protein -
LSI70_RS17525 (3382252) 3382252..3382737 - 486 WP_000214398.1 antirestriction protein -
LSI70_RS17530 (3382828) 3382828..3383646 - 819 WP_001234682.1 DUF932 domain-containing protein -
LSI70_RS17535 (3383986) 3383986..3385056 - 1071 WP_000102669.1 patatin-like phospholipase family protein -
LSI70_RS17540 (3385053) 3385053..3385958 - 906 WP_000203541.1 diguanylate cyclase regulator RdcB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T227507 WP_000854914.1 NZ_CP089034:c3380892-3380518 [Escherichia coli O157:H7]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T227507 NZ_CP122929:183501-183608 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13718.51 Da        Isoelectric Point: 6.6249

>AT227507 WP_001280954.1 NZ_CP089034:c3381313-3380939 [Escherichia coli O157:H7]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT227507 NZ_CP122929:c183453-183387 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0VS41

References