Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 414659..415459 Replicon chromosome
Accession NZ_CP089034
Organism Escherichia coli O157:H7 strain 6535NalR

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag LSI70_RS02025 Protein ID WP_000342448.1
Coordinates 414932..415459 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag LSI70_RS02020 Protein ID WP_001277108.1
Coordinates 414659..414925 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI70_RS02000 (410317) 410317..410985 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
LSI70_RS02005 (410978) 410978..412036 + 1059 WP_001042018.1 permease-like cell division protein FtsX -
LSI70_RS02010 (412281) 412281..413135 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
LSI70_RS02015 (413406) 413406..414509 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
LSI70_RS02020 (414659) 414659..414925 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
LSI70_RS02025 (414932) 414932..415459 + 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
LSI70_RS02030 (415456) 415456..415839 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
LSI70_RS02035 (416263) 416263..417372 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LSI70_RS02040 (417420) 417420..418346 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LSI70_RS02045 (418343) 418343..419620 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
LSI70_RS02050 (419617) 419617..420384 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T227491 WP_000342448.1 NZ_CP089034:414932-415459 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T227491 NZ_CP122923:2068857-2068960 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT227491 WP_001277108.1 NZ_CP089034:414659-414925 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT227491 NZ_CP122923:c2068961-2068727 [Escherichia coli]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References