Detailed information of TA system TA227489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 48879..49404
• Replicon: plasmid pO157
• Accession: NZ_CP089033
• Organism: Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: LSI69_RS28660
• Protein ID: WP_001159868.1
• Coordinates: 49099..49404 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: LSI69_RS28655
• Protein ID: WP_000813634.1
• Coordinates: 48879..49097 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI69_RS28625 (44162)	44162..44392	+	231	WP_010891288.1	hypothetical protein	-
LSI69_RS28630 (44513)	44513..45253	+	741	WP_001066920.1	tyrosine-type recombinase/integrase	-
LSI69_RS28635 (45538)	45538..46515	-	978	WP_000361615.1	RepB family plasmid replication initiator protein	-
LSI69_RS28640 (46923)	46923..47123	-	201	WP_000708307.1	hypothetical protein	-
LSI69_RS28645 (47120)	47120..47740	-	621	WP_001248529.1	hypothetical protein	-
LSI69_RS28650 (47737)	47737..48420	-	684	WP_010891291.1	DNA-binding protein	-
LSI69_RS28655 (48879)	48879..49097	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LSI69_RS28660 (49099)	49099..49404	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
LSI69_RS28665 (49405)	49405..50211	+	807	WP_000016989.1	site-specific integrase	-
LSI69_RS28675 (51866)	51866..52126	+	261	WP_071526583.1	RepB family plasmid replication initiator protein	-
LSI69_RS28680 (52714)	52714..53880	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB	1..92438	92438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T227489 WP_001159868.1 NZ_CP089033:49099-49404 [Escherichia coli O157:H7]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T227489 NZ_CP122923:939730-940137 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT227489 WP_000813634.1 NZ_CP089033:48879-49097 [Escherichia coli O157:H7]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT227489 NZ_CP122923:939483-939749 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2