Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1769..2328 Replicon plasmid pO157
Accession NZ_CP089033
Organism Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag LSI69_RS28435 Protein ID WP_000421248.1
Coordinates 2053..2328 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag LSI69_RS28430 Protein ID WP_001178089.1
Coordinates 1769..2053 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI69_RS28425 (1) 1..858 + 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
LSI69_RS28430 (1769) 1769..2053 + 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
LSI69_RS28435 (2053) 2053..2328 + 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LSI69_RS28440 (2423) 2423..2629 + 207 WP_001105064.1 hypothetical protein -
LSI69_RS28445 (2729) 2729..2944 - 216 Protein_5 transposase -
LSI69_RS28450 (2988) 2988..3554 - 567 Protein_6 IS3-like element IS600 family transposase -
LSI69_RS28455 (3554) 3554..3970 - 417 Protein_7 IS91 family transposase -
LSI69_RS28460 (3970) 3970..4155 - 186 WP_001302179.1 hypothetical protein -
LSI69_RS28465 (4332) 4332..6542 + 2211 WP_000592771.1 catalase/peroxidase KatP -
LSI69_RS28470 (6586) 6586..6975 + 390 WP_001172748.1 cytochrome b562 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92438 92438


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T227488 WP_000421248.1 NZ_CP089033:2053-2328 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T227488 NZ_CP122923:c842029-841652 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTTACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTTAC
CAGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACGCCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATATCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACAAGCCGGGATTC
AACGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTGAACAATATCACCCGGGGTAAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT227488 WP_001178089.1 NZ_CP089033:1769-2053 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT227488 NZ_CP122923:c842487-842119 [Escherichia coli]
GTGTCAGACACACTCCATGAAACAAATTATCCCGACGACAACAACGACCGCTCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCAGGGCACGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTCAGTCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References