Detailed information of TA system TA227486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 5102970..5103565
• Replicon: chromosome
• Accession: NZ_CP089032
• Organism: Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: LSI69_RS25870
• Protein ID: WP_000239579.1
• Coordinates: 5102970..5103320 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: LSI69_RS25875
• Protein ID: WP_001223210.1
• Coordinates: 5103314..5103565 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI69_RS25850 (5098416)	5098416..5099438	-	1023	WP_001301928.1	ABC transporter permease	-
LSI69_RS25855 (5099452)	5099452..5100954	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
LSI69_RS25860 (5101094)	5101094..5102050	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LSI69_RS25865 (5102360)	5102360..5102890	+	531	WP_000055075.1	inorganic diphosphatase	-
LSI69_RS25870 (5102970)	5102970..5103320	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
LSI69_RS25875 (5103314)	5103314..5103565	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LSI69_RS25880 (5103777)	5103777..5104118	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LSI69_RS25885 (5104121)	5104121..5107900	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T227486 WP_000239579.1 NZ_CP089032:c5103320-5102970 [Escherichia coli O157:H7]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T227486 NZ_CP122923:702019-702333 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT227486 WP_001223210.1 NZ_CP089032:c5103565-5103314 [Escherichia coli O157:H7]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT227486 NZ_CP122923:702330-702746 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8