Detailed information of TA system TA227483

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4853791..4854341
• Replicon: chromosome
• Accession: NZ_CP089032
• Organism: Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: LSI69_RS24820
• Protein ID: WP_001160975.1
• Coordinates: 4853791..4854105 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: LSI69_RS24825
• Protein ID: WP_000125566.1
• Coordinates: 4854108..4854341 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI69_RS24795 (4849466)	4849466..4850752	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
LSI69_RS24800 (4850752)	4850752..4851741	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
LSI69_RS24805 (4851738)	4851738..4852559	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
LSI69_RS24810 (4852562)	4852562..4852939	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
LSI69_RS24815 (4852946)	4852946..4853794	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
LSI69_RS24820 (4853791)	4853791..4854105	-	315	WP_001160975.1	CcdB family protein	Toxin
LSI69_RS24825 (4854108)	4854108..4854341	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
LSI69_RS24830 (4854427)	4854427..4854906	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
LSI69_RS24835 (4855098)	4855098..4856960	-	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
LSI69_RS24840 (4856953)	4856953..4857483	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
LSI69_RS24845 (4857591)	4857591..4858922	-	1332	WP_001183177.1	MFS transporter	-
LSI69_RS24850 (4858980)	4858980..4859267	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T227483 WP_001160975.1 NZ_CP089032:c4854105-4853791 [Escherichia coli O157:H7]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T227483 NZ_CP122885:43707-43967 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT227483 WP_000125566.1 NZ_CP089032:c4854341-4854108 [Escherichia coli O157:H7]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT227483 NZ_CP122885:43957-44208 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9