Detailed information of TA system TA022748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 122798..123488
• Replicon: plasmid pMRAD01
• Accession: NC_010510
• Organism: Methylobacterium radiotolerans JCM 2831

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MRAD2831_RS61025
• Protein ID: WP_012329620.1
• Coordinates: 123042..123488 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B1M8J7
• Locus tag: MRAD2831_RS61020
• Protein ID: WP_012329619.1
• Coordinates: 122798..123037 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRAD2831_RS60995	117864..119552	-	1689	WP_012329614.1	methyl-accepting chemotaxis protein	-
MRAD2831_RS61000	119799..119984	+	186	WP_012329615.1	hypothetical protein	-
MRAD2831_RS61005	120141..120416	+	276	WP_012329616.1	N-acetyltransferase	-
MRAD2831_RS61010	120492..121814	-	1323	WP_012329617.1	hypothetical protein	-
MRAD2831_RS61015	121903..122511	-	609	WP_012329618.1	PAS domain-containing protein	-
MRAD2831_RS61020	122798..123037	+	240	WP_012329619.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MRAD2831_RS61025	123042..123488	+	447	WP_012329620.1	type II toxin-antitoxin system VapC family toxin	Toxin
MRAD2831_RS61030	123534..124145	-	612	WP_012329621.1	TetR/AcrR family transcriptional regulator	-
MRAD2831_RS61035	124439..125524	+	1086	WP_012329622.1	platelet-activating factor acetylhydrolase plasma/intracellular isoform II	-
MRAD2831_RS61040	125597..126160	-	564	WP_012329623.1	TetR/AcrR family transcriptional regulator	-
MRAD2831_RS61045	126263..127468	+	1206	WP_012329624.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..586164	586164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15575.96 Da
• Isoelectric Point: 6.9677

>T22748 WP_012329620.1 NC_010510:123042-123488 [Methylobacterium radiotolerans JCM 2831]
VYLVDTNVLSAGAPTKAVPAVDLIDWMDRNSAGLHLSVVTIAEIEDGIAKSRREGAARKAERLAAWLETLLHLYAAKVLP
IDLAVARHIGGLSDLARGQGHAPGFADLAIAATARCNGWTILTRNVRHFAPLGVPACDPFVALPADGP

Nucleotide

• Length: 447 bp

>T22748 NC_010510:123042-123488 [Methylobacterium radiotolerans JCM 2831]
GTGTACCTCGTCGACACCAATGTCCTCTCGGCCGGCGCCCCGACCAAGGCCGTCCCGGCCGTCGACCTGATCGACTGGAT
GGACCGGAACAGCGCCGGGCTCCACCTCTCCGTCGTCACGATCGCCGAGATCGAGGACGGCATCGCGAAGAGCCGCAGGG
AGGGTGCCGCGCGCAAGGCTGAGCGCCTCGCGGCGTGGCTGGAGACCCTGCTGCACCTGTACGCCGCCAAGGTTCTCCCG
ATCGACCTCGCCGTCGCGCGGCACATCGGCGGATTGTCCGATCTGGCGCGCGGTCAGGGGCACGCGCCCGGCTTCGCGGA
TCTCGCGATCGCCGCCACGGCGCGCTGCAACGGCTGGACGATCCTCACTCGGAATGTGCGGCACTTCGCGCCGCTGGGCG
TTCCCGCGTGCGATCCCTTCGTGGCGCTGCCGGCCGATGGGCCGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8523.78 Da
• Isoelectric Point: 7.0109

>AT22748 WP_012329619.1 NC_010510:122798-123037 [Methylobacterium radiotolerans JCM 2831]
MQEIQLRDAKATLSAVIDQAKQGAPSIITRHGRPEAVLLSFEEWQRLSRVPSFGRLLMAAPVAADDLPSPSGGLRSAEL

Nucleotide

• Length: 240 bp

>AT22748 NC_010510:122798-123037 [Methylobacterium radiotolerans JCM 2831]
ATGCAGGAGATCCAGCTTCGCGACGCCAAGGCGACGCTGTCGGCCGTCATCGATCAGGCCAAGCAGGGCGCGCCCTCGAT
CATCACGCGCCACGGCCGGCCCGAGGCGGTGCTGCTCAGCTTCGAGGAGTGGCAGCGGCTGTCCCGCGTCCCGTCGTTCG
GCCGCCTGCTCATGGCCGCGCCCGTCGCGGCGGACGACCTCCCCTCCCCGTCCGGCGGGCTCAGAAGCGCCGAGCTGTGA

Structures

Antitoxin

• AlphaFold DB: B1M8J7