Detailed information of TA system TA227479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3692703..3693181
• Replicon: chromosome
• Accession: NZ_CP089032
• Organism: Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: LSI69_RS19295
• Protein ID: WP_001303876.1
• Coordinates: 3692894..3693181 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: LSI69_RS19290
• Protein ID: WP_000536233.1
• Coordinates: 3692703..3692894 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSI69_RS19255 (3688107)	3688107..3688877	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
LSI69_RS19260 (3688899)	3688899..3689645	-	747	WP_000788751.1	ATP-binding protein	-
LSI69_RS19265 (3689652)	3689652..3690743	-	1092	WP_001205823.1	hypothetical protein	-
LSI69_RS19270 (3690822)	3690822..3691277	+	456	WP_000273724.1	hypothetical protein	-
LSI69_RS19275 (3691484)	3691484..3691909	-	426	WP_000693855.1	toxin YdaT family protein	-
LSI69_RS19280 (3691893)	3691893..3692165	-	273	WP_000887453.1	YdaS family helix-turn-helix protein	-
LSI69_RS19285 (3692274)	3692274..3692675	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
LSI69_RS19290 (3692703)	3692703..3692894	+	192	WP_000536233.1	hypothetical protein	Antitoxin
LSI69_RS19295 (3692894)	3692894..3693181	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSI69_RS19300 (3693459)	3693459..3693614	+	156	WP_000379575.1	DUF1391 family protein	-
LSI69_RS28995 (3693616)	3693616..3693744	+	129	WP_000344963.1	protein YdfB	-
LSI69_RS19305 (3693756)	3693756..3694145	+	390	WP_000394511.1	hypothetical protein	-
LSI69_RS19310 (3694332)	3694332..3694517	-	186	WP_001133046.1	hypothetical protein	-
LSI69_RS19315 (3695091)	3695091..3695279	+	189	WP_000413705.1	cell division inhibition protein DicB	-
LSI69_RS19320 (3695276)	3695276..3695467	+	192	WP_001098307.1	DUF1482 family protein	-
LSI69_RS19325 (3695561)	3695561..3698032	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T227479 WP_001303876.1 NZ_CP089032:3692894-3693181 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T227479 NZ_CP122882:c18378-17998 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT227479 WP_000536233.1 NZ_CP089032:3692703-3692894 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT227479 NZ_CP122882:c18599-18378 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA