Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2787583..2788221 Replicon chromosome
Accession NZ_CP089032
Organism Escherichia coli O157:H7 strain 6535WT

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag LSI69_RS14105 Protein ID WP_000813794.1
Coordinates 2788045..2788221 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LSI69_RS14100 Protein ID WP_001270286.1
Coordinates 2787583..2787999 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSI69_RS14080 (2782735) 2782735..2783676 - 942 WP_001251320.1 ABC transporter permease -
LSI69_RS14085 (2783677) 2783677..2784690 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
LSI69_RS14090 (2784708) 2784708..2785853 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
LSI69_RS14095 (2786098) 2786098..2787504 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
LSI69_RS14100 (2787583) 2787583..2787999 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LSI69_RS14105 (2788045) 2788045..2788221 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LSI69_RS14110 (2788443) 2788443..2788673 + 231 WP_000494244.1 YncJ family protein -
LSI69_RS14115 (2788765) 2788765..2790726 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LSI69_RS14120 (2790799) 2790799..2791335 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
LSI69_RS14125 (2791388) 2791388..2792599 + 1212 WP_071525382.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T227477 WP_000813794.1 NZ_CP089032:c2788221-2788045 [Escherichia coli O157:H7]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T227477 NZ_CP122880:c4574370-4574197 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT227477 WP_001270286.1 NZ_CP089032:c2787999-2787583 [Escherichia coli O157:H7]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT227477 NZ_CP122880:c4574694-4574398 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References