Detailed information of TA system TA022747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 853896..854428
• Replicon: chromosome
• Accession: NC_010508
• Organism: Burkholderia cenocepacia MC0-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1JWG6
• Locus tag: BCENMC03_RS03865
• Protein ID: WP_011544674.1
• Coordinates: 853896..854219 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BCENMC03_RS35840
• Protein ID: WP_012327974.1
• Coordinates: 854216..854428 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCENMC03_RS03835	848909..849742	-	834	WP_041492055.1	class II glutamine amidotransferase	-
BCENMC03_RS03840	849937..850266	-	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
BCENMC03_RS03845	850396..851343	+	948	WP_012327970.1	AraC family transcriptional regulator	-
BCENMC03_RS03850	851447..851890	+	444	WP_012327971.1	YiaA/YiaB family protein	-
BCENMC03_RS35830	852013..853047	-	1035	WP_012327972.1	relaxase/mobilization nuclease domain-containing protein	-
BCENMC03_RS35835	853049..853573	-	525	WP_012327973.1	hypothetical protein	-
BCENMC03_RS03865	853896..854219	-	324	WP_011544674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BCENMC03_RS35840	854216..854428	-	213	WP_012327974.1	antitoxin MazE family protein	Antitoxin
BCENMC03_RS03870	854540..855019	-	480	WP_012327975.1	hypothetical protein	-
BCENMC03_RS03875	855087..855482	-	396	WP_012327976.1	hypothetical protein	-
BCENMC03_RS03880	855623..855943	+	321	WP_012327977.1	helix-turn-helix domain-containing protein	-
BCENMC03_RS03890	856373..857122	-	750	WP_011544679.1	tol-pal system protein YbgF	-
BCENMC03_RS03895	857142..857654	-	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
BCENMC03_RS03900	857672..858967	-	1296	WP_012327978.1	Tol-Pal system protein TolB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11668.77 Da
• Isoelectric Point: 8.9366

>T22747 WP_011544674.1 NC_010508:c854219-853896 [Burkholderia cenocepacia MC0-3]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSSMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T22747 NC_010508:c854219-853896 [Burkholderia cenocepacia MC0-3]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACACCCCAGTGTAACGTTGCTGCTAATGTCGAGCTCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACTGTGCGCCGCGAAAAA
ATCGGTCGGCCCATCGGCCACCTCGAAGACGAACTGATGGTGGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7997.94 Da
• Isoelectric Point: 5.5492

>AT22747 WP_012327974.1 NC_010508:c854428-854216 [Burkholderia cenocepacia MC0-3]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQATDLGHSQ

Nucleotide

• Length: 213 bp

>AT22747 NC_010508:c854428-854216 [Burkholderia cenocepacia MC0-3]
ATGGCAGCTACCACTTCCGAGCGGGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGTCCAATTCAGATATG
GGTACCGGACGTCCGCCAGCCGGGTTTCGCCGATGAATGCGCTCGACAATCGCGCATGGTCCATCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B1JWG6