Detailed information of TA system TA227459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4837027..4837622
• Replicon: chromosome
• Accession: NZ_CP089031
• Organism: Escherichia coli strain 7386Nal

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: CO543_RS24235
• Protein ID: WP_000239579.1
• Coordinates: 4837027..4837377 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: CO543_RS24240
• Protein ID: WP_001223210.1
• Coordinates: 4837371..4837622 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO543_RS24215 (4832473)	4832473..4833495	-	1023	WP_001301928.1	ABC transporter permease	-
CO543_RS24220 (4833509)	4833509..4835011	-	1503	Protein_4759	sugar ABC transporter ATP-binding protein	-
CO543_RS24225 (4835151)	4835151..4836107	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
CO543_RS24230 (4836417)	4836417..4836947	+	531	WP_000055075.1	inorganic diphosphatase	-
CO543_RS24235 (4837027)	4837027..4837377	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
CO543_RS24240 (4837371)	4837371..4837622	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
CO543_RS24245 (4837834)	4837834..4838175	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
CO543_RS24250 (4838178)	4838178..4841957	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T227459 WP_000239579.1 NZ_CP089031:c4837377-4837027 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T227459 NZ_CP122880:651876-652190 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT227459 WP_001223210.1 NZ_CP089031:c4837622-4837371 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT227459 NZ_CP122880:652187-652603 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8