Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-HTH_XRE
Location 3637917..3638622 Replicon chromosome
Accession NZ_CP089031
Organism Escherichia coli strain 7386Nal

Toxin (Protein)


Gene name relE Uniprot ID Q8X3N0
Locus tag CO543_RS18770 Protein ID WP_000539519.1
Coordinates 3637917..3638303 (+) Length 129 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag CO543_RS18775 Protein ID WP_001280945.1
Coordinates 3638293..3638622 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO543_RS18750 (3633921) 3633921..3634547 + 627 WP_001295292.1 glutathione S-transferase GstB -
CO543_RS18755 (3634544) 3634544..3635659 - 1116 WP_000554959.1 aldose sugar dehydrogenase YliI -
CO543_RS18760 (3635770) 3635770..3636153 - 384 WP_000497137.1 biofilm formation regulator BssR -
CO543_RS18765 (3636366) 3636366..3637691 + 1326 WP_000049367.1 30S ribosomal protein S12 methylthiotransferase RimO -
CO543_RS18770 (3637917) 3637917..3638303 + 387 WP_000539519.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CO543_RS18775 (3638293) 3638293..3638622 + 330 WP_001280945.1 DNA-binding transcriptional regulator Antitoxin
CO543_RS18780 (3638692) 3638692..3640020 - 1329 WP_000086905.1 GGDEF domain-containing protein -
CO543_RS18785 (3640028) 3640028..3642376 - 2349 WP_000950320.1 EAL domain-containing protein -
CO543_RS18790 (3642554) 3642554..3643465 - 912 WP_001236044.1 glutathione ABC transporter permease GsiD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(1-124)

Antitoxin

Cro/C1-type helix-turn-helix domain

(53-94)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14279.42 Da        Isoelectric Point: 9.9296

>T227453 WP_000539519.1 NZ_CP089031:3637917-3638303 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGSKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Download         Length: 387 bp

>T227453 NZ_CP122877:c20753-20505 [Escherichia coli]
ATGAGAAAAAATAAAGGCGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCTGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11982.08 Da        Isoelectric Point: 10.0101

>AT227453 WP_001280945.1 NZ_CP089031:3638293-3638622 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Download         Length: 330 bp

>AT227453 NZ_CP122877:c20515-20288 [Escherichia coli]
ATGAAACCTGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References