Detailed information of TA system TA227452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3471777..3472255
• Replicon: chromosome
• Accession: NZ_CP089031
• Organism: Escherichia coli strain 7386Nal

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: CO543_RS18025
• Protein ID: WP_001303876.1
• Coordinates: 3471968..3472255 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: CO543_RS18020
• Protein ID: WP_000536233.1
• Coordinates: 3471777..3471968 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO543_RS17985 (3467175)	3467175..3467945	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
CO543_RS17990 (3467967)	3467967..3468713	-	747	WP_000788745.1	ATP-binding protein	-
CO543_RS17995 (3468720)	3468720..3469817	-	1098	WP_001475117.1	hypothetical protein	-
CO543_RS18000 (3469896)	3469896..3470351	+	456	WP_000273724.1	hypothetical protein	-
CO543_RS18005 (3470558)	3470558..3470983	-	426	WP_000693855.1	toxin YdaT family protein	-
CO543_RS18010 (3470967)	3470967..3471239	-	273	WP_000887453.1	YdaS family helix-turn-helix protein	-
CO543_RS18015 (3471348)	3471348..3471749	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
CO543_RS18020 (3471777)	3471777..3471968	+	192	WP_000536233.1	hypothetical protein	Antitoxin
CO543_RS18025 (3471968)	3471968..3472255	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO543_RS18030 (3472533)	3472533..3472688	+	156	WP_000379575.1	DUF1391 family protein	-
CO543_RS26980 (3472690)	3472690..3472818	+	129	WP_000344963.1	protein YdfB	-
CO543_RS18035 (3472830)	3472830..3473219	+	390	WP_000394511.1	hypothetical protein	-
CO543_RS18040 (3473406)	3473406..3473591	-	186	WP_001133046.1	hypothetical protein	-
CO543_RS18045 (3474165)	3474165..3474353	+	189	WP_000413705.1	cell division inhibition protein DicB	-
CO543_RS18050 (3474350)	3474350..3474541	+	192	WP_001098307.1	DUF1482 family protein	-
CO543_RS18055 (3474635)	3474635..3477106	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T227452 WP_001303876.1 NZ_CP089031:3471968-3472255 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T227452 NZ_CP122877:c18786-18538 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACAGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT227452 WP_000536233.1 NZ_CP089031:3471777-3471968 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT227452 NZ_CP122877:c18541-18194 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAATTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA