Detailed information of TA system TA022744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5540226..5541768
• Replicon: chromosome
• Accession: NC_010506
• Organism: Shewanella woodyi ATCC 51908

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B1KL15
• Locus tag: SWOO_RS23450
• Protein ID: WP_012327146.1
• Coordinates: 5540461..5541768 (+)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: SWOO_RS23445
• Protein ID: WP_012327145.1
• Coordinates: 5540226..5540474 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SWOO_RS23425	5536447..5537106	+	660	WP_012327141.1	GTP cyclohydrolase I FolE	-
SWOO_RS23430	5537355..5537996	-	642	WP_012327142.1	orotate phosphoribosyltransferase	-
SWOO_RS23435	5538084..5538797	-	714	WP_012327143.1	ribonuclease PH	-
SWOO_RS23440	5539010..5539873	+	864	WP_012327144.1	YicC family protein	-
SWOO_RS23445	5540226..5540474	+	249	WP_012327145.1	helix-turn-helix domain-containing protein	Antitoxin
SWOO_RS23450	5540461..5541768	+	1308	WP_012327146.1	HipA domain-containing protein	Toxin
SWOO_RS23455	5541796..5542185	+	390	Protein_4556	DUF1732 domain-containing protein	-
SWOO_RS23460	5542356..5543612	-	1257	WP_012327148.1	type II toxin-antitoxin system HipA family toxin	-
SWOO_RS23465	5543609..5543923	-	315	WP_041417840.1	helix-turn-helix domain-containing protein	-
SWOO_RS23470	5544346..5545038	-	693	WP_195743010.1	amino acid adenylation	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (146-387)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-65)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 49169.18 Da
• Isoelectric Point: 5.3217

>T22744 WP_012327146.1 NC_010506:5540461-5541768 [Shewanella woodyi ATCC 51908]
MSGSNFHTLEMHFRGRVIGTIGFDIDTERFSIEYTKAWQENGFPISPKLPLDGQANSSQISIYLTNLLPENKGLDHLIEA
LGIAKSNTFALIKSIGFDTSGAILYLPVATKPPVTSFTPISKADLIKRIEDPEHWPMEVWDGKPRLSVAGVQSKLNLFHT
DTEYGFGEGELCSTHIIKFEKSNQQHLVLNEFMTMTLADLIGMQVAKVEILPIGKYKALSVTRFDRMYDPERHIVMRRHQ
IDACQALGFSVARKYERYLGSGRDVRDIREGVSLEKLFSIAEHCANPAQAKFDMLRWTIFNLLVCNADSHGKNYSFYVTP
NGLEPTPWYDLVNVQMYPDFDQELAMAIGDEFEADKIHAYQLMTFAEECGIKQNILELTLKQLAKAIIGNLDKAANQAGE
LTDAESEYVGAYKATIQARCEYYLTQVEEMKHIEL

Nucleotide

• Length: 1308 bp

>T22744 NC_010506:5540461-5541768 [Shewanella woodyi ATCC 51908]
ATGAGTGGTTCTAACTTTCACACACTTGAGATGCACTTTAGAGGCAGGGTTATTGGGACGATAGGTTTTGACATAGACAC
TGAACGATTCTCTATCGAATACACTAAAGCTTGGCAGGAAAATGGTTTTCCTATTTCACCAAAGCTCCCACTTGATGGCC
AAGCTAATAGTTCTCAAATTTCCATTTATCTGACTAACCTACTGCCCGAAAATAAAGGGCTTGATCACCTTATTGAAGCC
CTAGGTATTGCCAAAAGTAATACATTTGCTTTGATCAAAAGCATTGGATTCGATACCTCAGGTGCAATATTGTATCTGCC
TGTGGCCACCAAGCCACCAGTAACCTCTTTTACCCCAATTAGTAAAGCAGATCTTATCAAGCGAATCGAAGATCCTGAAC
ATTGGCCGATGGAAGTCTGGGACGGAAAACCACGCCTTTCCGTTGCAGGGGTTCAATCAAAATTAAACCTGTTCCACACC
GACACAGAATACGGATTTGGTGAAGGTGAGCTGTGTTCAACCCACATCATTAAATTTGAAAAAAGCAATCAGCAGCACCT
CGTACTCAACGAATTTATGACCATGACACTTGCCGACCTCATTGGTATGCAGGTGGCCAAAGTAGAGATATTGCCAATAG
GGAAATACAAAGCGTTATCCGTCACCCGCTTCGATCGCATGTACGATCCTGAGCGTCATATTGTAATGCGTCGTCATCAG
ATCGATGCATGTCAAGCTCTGGGCTTTTCCGTCGCAAGAAAGTATGAACGATATCTTGGTAGTGGTCGTGACGTACGAGA
CATCCGTGAAGGAGTAAGCCTAGAAAAACTGTTTAGTATCGCAGAGCACTGCGCTAACCCTGCACAAGCCAAGTTTGACA
TGCTTCGCTGGACAATCTTTAACTTACTCGTCTGCAATGCGGATTCACATGGCAAAAACTATTCATTCTATGTCACTCCG
AATGGGCTTGAACCGACCCCATGGTATGATCTTGTCAACGTGCAGATGTACCCTGATTTTGATCAAGAGCTTGCTATGGC
TATTGGGGATGAGTTCGAGGCTGATAAGATCCACGCCTACCAATTAATGACATTTGCTGAAGAGTGCGGCATTAAGCAAA
ACATACTTGAATTAACCCTCAAGCAACTAGCGAAAGCAATTATCGGTAACCTGGATAAAGCGGCAAACCAAGCTGGGGAG
CTTACGGATGCAGAATCCGAGTATGTTGGAGCTTACAAAGCAACGATTCAAGCACGATGTGAATACTACCTAACTCAGGT
TGAAGAAATGAAGCACATTGAGCTATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9133.56 Da
• Isoelectric Point: 10.3478

>AT22744 WP_012327145.1 NC_010506:5540226-5540474 [Shewanella woodyi ATCC 51908]
MVTQLNPNTLGKRIKDRRKAANMTQEVAASFCGVTKKTLIRVEKGEDVYFSTIVKILNGLGIKLTFDTPNSENKLGGSNE
WF

Nucleotide

• Length: 249 bp

>AT22744 NC_010506:5540226-5540474 [Shewanella woodyi ATCC 51908]
ATGGTCACTCAACTCAATCCAAACACTTTAGGCAAACGAATCAAAGACCGCCGAAAGGCCGCGAACATGACTCAGGAAGT
AGCCGCATCATTCTGTGGTGTTACCAAGAAGACCTTGATCAGAGTCGAAAAAGGCGAAGATGTATACTTTTCAACGATAG
TGAAAATCCTCAATGGCCTTGGTATAAAGTTGACCTTCGATACTCCCAATAGCGAAAACAAATTAGGTGGCAGTAATGAG
TGGTTCTAA

Structures

Toxin

• AlphaFold DB: B1KL15