Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2480257..2480895 Replicon chromosome
Accession NZ_CP089031
Organism Escherichia coli strain 7386Nal

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag CO543_RS12685 Protein ID WP_000813794.1
Coordinates 2480257..2480433 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag CO543_RS12690 Protein ID WP_001270286.1
Coordinates 2480479..2480895 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CO543_RS12665 (2475878) 2475878..2477051 - 1174 Protein_2485 BenE family transporter YdcO -
CO543_RS12670 (2477143) 2477143..2477679 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
CO543_RS12675 (2477752) 2477752..2479713 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
CO543_RS12680 (2479805) 2479805..2480035 - 231 WP_000494244.1 YncJ family protein -
CO543_RS12685 (2480257) 2480257..2480433 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
CO543_RS12690 (2480479) 2480479..2480895 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
CO543_RS12695 (2480974) 2480974..2482380 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
CO543_RS12700 (2482625) 2482625..2483770 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
CO543_RS12705 (2483788) 2483788..2484801 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
CO543_RS12710 (2484802) 2484802..2485743 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T227437 WP_000813794.1 NZ_CP089031:2480257-2480433 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T227437 NZ_CP122874:74700-75098 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT227437 WP_001270286.1 NZ_CP089031:2480479-2480895 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT227437 NZ_CP122874:74473-74700 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References