Detailed information of TA system TA227426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 156286..156956
• Replicon: plasmid p49210-229.3
• Accession: NZ_CP089030
• Organism: Klebsiella pneumoniae strain 49210

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LRY78_RS29340
• Protein ID: WP_004213072.1
• Coordinates: 156286..156729 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRY78_RS29345
• Protein ID: WP_004213073.1
• Coordinates: 156726..156956 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRY78_RS29305 (LRY78_29295)	151698..151973	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRY78_RS29310 (LRY78_29300)	152036..152527	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRY78_RS29315 (LRY78_29305)	152576..153496	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LRY78_RS29320 (LRY78_29310)	153587..153990	+	404	Protein_168	GAF domain-containing protein	-
LRY78_RS29325 (LRY78_29315)	154508..155142	-	635	Protein_169	mucoid phenotype regulator RmpA2	-
LRY78_RS29330 (LRY78_29320)	155559..155863	+	305	Protein_170	transposase	-
LRY78_RS29335 (LRY78_29325)	155886..156137	-	252	WP_186987481.1	hypothetical protein	-
LRY78_RS29340 (LRY78_29330)	156286..156729	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRY78_RS29345 (LRY78_29335)	156726..156956	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRY78_RS29350 (LRY78_29340)	157564..158697	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRY78_RS29355 (LRY78_29345)	158713..159006	+	294	WP_004213076.1	hypothetical protein	-
LRY78_RS29360 (LRY78_29350)	158996..159202	-	207	WP_004213077.1	hypothetical protein	-
LRY78_RS29365 (LRY78_29355)	159554..159844	+	291	WP_004213078.1	hypothetical protein	-
LRY78_RS29370 (LRY78_29360)	159834..160733	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..229288	229288

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227426 WP_004213072.1 NZ_CP089030:c156729-156286 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227426 NZ_CP122872:1012090-1012425 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227426 WP_004213073.1 NZ_CP089030:c156956-156726 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227426 NZ_CP122872:1011842-1012090 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620