Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 103643..104553 Replicon plasmid p49210-229.3
Accession NZ_CP089030
Organism Klebsiella pneumoniae strain 49210

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LRY78_RS29040 Protein ID WP_004026354.1
Coordinates 103643..104113 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag LRY78_RS29045 Protein ID WP_004026357.1
Coordinates 104110..104553 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRY78_RS29015 (LRY78_29005) 99055..99453 - 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -
LRY78_RS29020 (LRY78_29010) 99790..100284 - 495 WP_004212794.1 thermonuclease family protein -
LRY78_RS29025 (LRY78_29015) 100462..100728 + 267 WP_223175074.1 DUF1173 family protein -
LRY78_RS29030 (LRY78_29020) 100761..101411 + 651 WP_068893702.1 DUF1173 family protein -
LRY78_RS29040 (LRY78_29030) 103643..104113 - 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LRY78_RS29045 (LRY78_29035) 104110..104553 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
LRY78_RS29050 (LRY78_29040) 104654..105157 - 504 Protein_114 DUF4113 domain-containing protein -
LRY78_RS29055 (LRY78_29045) 105255..105476 - 222 WP_004213643.1 DUF2188 domain-containing protein -
LRY78_RS29060 (LRY78_29050) 105651..105776 - 126 Protein_116 Fe3+-citrate ABC transporter substrate-binding protein -
LRY78_RS29065 (LRY78_29055) 105851..107977 - 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LRY78_RS29070 (LRY78_29060) 108064..109017 - 954 Protein_118 fec operon regulator FecR -
LRY78_RS29075 (LRY78_29065) 109014..109535 - 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA 1..229288 229288


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T227425 WP_004026354.1 NZ_CP089030:c104113-103643 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T227425 NZ_CP122872:913974-914381 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT227425 WP_004026357.1 NZ_CP089030:c104553-104110 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT227425 NZ_CP122872:913727-913993 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References