Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53023..53548 Replicon plasmid p49210-124.9
Accession NZ_CP089029
Organism Klebsiella pneumoniae strain 49210

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag LRY78_RS28025 Protein ID WP_004197633.1
Coordinates 53243..53548 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag LRY78_RS28020 Protein ID WP_001568025.1
Coordinates 53023..53241 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRY78_RS27975 (LRY78_27965) 48129..49124 + 996 WP_019725651.1 hypothetical protein -
LRY78_RS27980 (LRY78_27970) 49197..49520 + 324 WP_019725650.1 hypothetical protein -
LRY78_RS27985 (LRY78_27975) 49954..50148 + 195 WP_019725033.1 hypothetical protein -
LRY78_RS27990 (LRY78_27980) 50192..50422 - 231 WP_019725034.1 hypothetical protein -
LRY78_RS27995 (LRY78_27985) 50436..50639 - 204 WP_019725040.1 HHA domain-containing protein -
LRY78_RS28000 (LRY78_27990) 50673..51041 - 369 WP_015065502.1 hypothetical protein -
LRY78_RS28005 (LRY78_27995) 51085..51579 - 495 WP_009310053.1 hypothetical protein -
LRY78_RS28010 (LRY78_28000) 51610..52182 - 573 WP_015065504.1 hypothetical protein -
LRY78_RS28015 (LRY78_28005) 52179..52427 - 249 WP_019725036.1 hypothetical protein -
LRY78_RS28020 (LRY78_28010) 53023..53241 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
LRY78_RS28025 (LRY78_28015) 53243..53548 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
LRY78_RS28030 (LRY78_28020) 53717..54118 + 402 WP_073558206.1 hypothetical protein -
LRY78_RS28035 (LRY78_28025) 54145..54468 + 324 WP_004197641.1 hypothetical protein -
LRY78_RS28040 (LRY78_28030) 54465..55481 + 1017 WP_004197639.1 hypothetical protein -
LRY78_RS28045 (LRY78_28035) 55679..56473 + 795 WP_004197635.1 site-specific integrase -
LRY78_RS28050 (LRY78_28040) 56913..57215 - 303 WP_004197636.1 hypothetical protein -
LRY78_RS28055 (LRY78_28045) 57212..57838 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / tet(A) / qnrB1 / dfrA14 / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 - 1..124933 124933


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T227423 WP_004197633.1 NZ_CP089029:53243-53548 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T227423 NZ_CP122872:707374-707688 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT227423 WP_001568025.1 NZ_CP089029:53023-53241 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT227423 NZ_CP122872:707685-708101 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References