Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5385151..5385776 Replicon chromosome
Accession NZ_CP089024
Organism Klebsiella pneumoniae strain 49210

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag LRY78_RS26235 Protein ID WP_012737091.1
Coordinates 5385151..5385534 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRY78_RS26240 Protein ID WP_004150355.1
Coordinates 5385534..5385776 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRY78_RS26220 (5382517) 5382517..5383419 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRY78_RS26225 (5383416) 5383416..5384051 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRY78_RS26230 (5384048) 5384048..5384977 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRY78_RS26235 (5385151) 5385151..5385534 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
LRY78_RS26240 (5385534) 5385534..5385776 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRY78_RS26245 (5385981) 5385981..5386898 + 918 WP_012737090.1 alpha/beta hydrolase -
LRY78_RS26250 (5386912) 5386912..5387853 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRY78_RS26255 (5387898) 5387898..5388335 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRY78_RS26260 (5388332) 5388332..5389192 - 861 WP_004146232.1 virulence factor BrkB family protein -
LRY78_RS26265 (5389186) 5389186..5389785 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T227421 WP_012737091.1 NZ_CP089024:c5385534-5385151 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227421 NZ_CP122872:210208-210315 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227421 WP_004150355.1 NZ_CP089024:c5385776-5385534 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227421 NZ_CP122872:c210160-210094 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References