Detailed information of TA system TA227405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 154931..155601
• Replicon: plasmid p53370-227.9
• Accession: NZ_CP089023
• Organism: Klebsiella pneumoniae strain 53370

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LRZ76_RS27745
• Protein ID: WP_004213072.1
• Coordinates: 154931..155374 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRZ76_RS27750
• Protein ID: WP_004213073.1
• Coordinates: 155371..155601 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ76_RS27710 (LRZ76_27710)	150342..150617	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRZ76_RS27715 (LRZ76_27715)	150680..151171	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRZ76_RS27720 (LRZ76_27720)	151220..152140	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LRZ76_RS27725 (LRZ76_27725)	152231..152634	+	404	Protein_166	GAF domain-containing protein	-
LRZ76_RS27730 (LRZ76_27730)	153152..153787	-	636	Protein_167	mucoid phenotype regulator RmpA2	-
LRZ76_RS27735 (LRZ76_27735)	154204..154508	+	305	Protein_168	transposase	-
LRZ76_RS27740 (LRZ76_27740)	154531..154782	-	252	WP_186987481.1	hypothetical protein	-
LRZ76_RS27745 (LRZ76_27745)	154931..155374	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ76_RS27750 (LRZ76_27750)	155371..155601	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ76_RS27755 (LRZ76_27755)	156209..157342	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRZ76_RS27760 (LRZ76_27760)	157358..157651	+	294	WP_004213076.1	hypothetical protein	-
LRZ76_RS27765 (LRZ76_27765)	157641..157847	-	207	WP_226323723.1	hypothetical protein	-
LRZ76_RS27770 (LRZ76_27770)	158199..158489	+	291	WP_004213078.1	hypothetical protein	-
LRZ76_RS27775 (LRZ76_27775)	158479..159378	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..227890	227890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227405 WP_004213072.1 NZ_CP089023:c155374-154931 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227405 NZ_CP122868:1830581-1830832 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227405 WP_004213073.1 NZ_CP089023:c155601-155371 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227405 NZ_CP122868:1830829-1831083 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620