Detailed information of TA system TA227401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 35866..36401
• Replicon: plasmid p53370-97.9
• Accession: NZ_CP089022
• Organism: Klebsiella pneumoniae strain 53370

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LRZ76_RS26560
• Protein ID: WP_016531292.1
• Coordinates: 35866..36153 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A837JUA1
• Locus tag: LRZ76_RS26565
• Protein ID: WP_023317799.1
• Coordinates: 36150..36401 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ76_RS26540 (LRZ76_26540)	31309..32808	-	1500	WP_004883360.1	IS21 family transposase	-
LRZ76_RS26545 (LRZ76_26545)	32995..33525	+	531	WP_023317796.1	hypothetical protein	-
LRZ76_RS26550 (LRZ76_26550)	33779..34066	-	288	WP_016529486.1	hypothetical protein	-
LRZ76_RS26555 (LRZ76_26555)	34156..34818	-	663	WP_023317797.1	division plane positioning ATPase MipZ	-
LRZ76_RS26560 (LRZ76_26560)	35866..36153	-	288	WP_016531292.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRZ76_RS26565 (LRZ76_26565)	36150..36401	-	252	WP_023317799.1	hypothetical protein	Antitoxin
LRZ76_RS26570 (LRZ76_26570)	36497..37765	-	1269	WP_023317800.1	Y-family DNA polymerase	-
LRZ76_RS26575 (LRZ76_26575)	37765..38196	-	432	WP_023317801.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LRZ76_RS26580 (LRZ76_26580)	38602..40089	+	1488	WP_161952885.1	group II intron reverse transcriptase/maturase	-
LRZ76_RS26585 (LRZ76_26585)	40338..41309	+	972	WP_023317803.1	plasmid segregation protein ParM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB4 / blaDHA-1	-	1..97945	97945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11120.16 Da
• Isoelectric Point: 10.4373

>T227401 WP_016531292.1 NZ_CP089022:c36153-35866 [Klebsiella pneumoniae]
MTYTVKFREDALKEWNKLDKTIQQQFAKKLKKCCENPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDNQLIIAIVAVGK
RERSDVYTLASERMK

Nucleotide

• Length: 288 bp

>T227401 NZ_CP122868:c619033-618569 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9239.60 Da
• Isoelectric Point: 4.2108

>AT227401 WP_023317799.1 NZ_CP089022:c36401-36150 [Klebsiella pneumoniae]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELIKLVAERSNQPLFDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT227401 NZ_CP122868:c619368-619033 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
ACCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A837JUA1