Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5401150..5401775 Replicon chromosome
Accession NZ_CP089021
Organism Klebsiella pneumoniae strain 53370

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag LRZ76_RS25935 Protein ID WP_012737091.1
Coordinates 5401150..5401533 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRZ76_RS25940 Protein ID WP_004150355.1
Coordinates 5401533..5401775 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ76_RS25920 (5398516) 5398516..5399418 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRZ76_RS25925 (5399415) 5399415..5400050 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRZ76_RS25930 (5400047) 5400047..5400976 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRZ76_RS25935 (5401150) 5401150..5401533 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ76_RS25940 (5401533) 5401533..5401775 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRZ76_RS25945 (5401980) 5401980..5402897 + 918 WP_012737090.1 alpha/beta hydrolase -
LRZ76_RS25950 (5402911) 5402911..5403852 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRZ76_RS25955 (5403897) 5403897..5404334 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRZ76_RS25960 (5404331) 5404331..5405191 - 861 WP_004146232.1 virulence factor BrkB family protein -
LRZ76_RS25965 (5405185) 5405185..5405784 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T227400 WP_012737091.1 NZ_CP089021:c5401533-5401150 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227400 NZ_CP122868:314675-315202 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227400 WP_004150355.1 NZ_CP089021:c5401775-5401533 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227400 NZ_CP122868:314402-314668 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References