Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4814570..4815092 Replicon chromosome
Accession NC_010498
Organism Escherichia coli SMS-3-5

Toxin (Protein)


Gene name relE Uniprot ID V0SDB6
Locus tag ECSMS35_RS23705 Protein ID WP_001105432.1
Coordinates 4814802..4815092 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag ECSMS35_RS23700 Protein ID WP_000212715.1
Coordinates 4814570..4814812 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSMS35_RS23680 4810483..4811856 + 1374 WP_001219785.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
ECSMS35_RS23685 4811939..4812490 - 552 WP_000166270.1 ribosome-associated protein -
ECSMS35_RS23690 4812584..4813936 + 1353 WP_001162169.1 metalloprotease PmbA -
ECSMS35_RS23695 4813992..4814378 + 387 WP_001232253.1 cytochrome b562 -
ECSMS35_RS23700 4814570..4814812 + 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
ECSMS35_RS23705 4814802..4815092 + 291 WP_001105432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECSMS35_RS23710 4815093..4815557 - 465 WP_001009180.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
ECSMS35_RS23715 4815742..4817880 - 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
ECSMS35_RS23725 4818274..4819929 - 1656 WP_012311860.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-82)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11311.21 Da        Isoelectric Point: 10.0238

>T22740 WP_001105432.1 NC_010498:4814802-4815092 [Escherichia coli SMS-3-5]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTLGYRLVYSVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T22740 NC_010498:4814802-4815092 [Escherichia coli SMS-3-5]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT22740 WP_000212715.1 NC_010498:4814570-4814812 [Escherichia coli SMS-3-5]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT22740 NC_010498:4814570-4814812 [Escherichia coli SMS-3-5]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SDB6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References