Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4763882..4764692 Replicon chromosome
Accession NZ_CP089021
Organism Klebsiella pneumoniae strain 53370

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag LRZ76_RS22965 Protein ID WP_014908042.1
Coordinates 4763882..4764415 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LRZ76_RS22970 Protein ID WP_002887278.1
Coordinates 4764426..4764692 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ76_RS22960 (4762713) 4762713..4763834 + 1122 WP_012737335.1 cupin domain-containing protein -
LRZ76_RS22965 (4763882) 4763882..4764415 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
LRZ76_RS22970 (4764426) 4764426..4764692 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LRZ76_RS22975 (4764795) 4764795..4766228 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
LRZ76_RS22980 (4766218) 4766218..4766901 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LRZ76_RS22985 (4767073) 4767073..4768458 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
LRZ76_RS22990 (4768476) 4768476..4768820 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T227398 WP_014908042.1 NZ_CP089021:c4764415-4763882 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T227398 NZ_CP122868:218489-218596 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT227398 WP_002887278.1 NZ_CP089021:c4764692-4764426 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT227398 NZ_CP122868:c218440-218377 [Escherichia coli]
GTCTAGATGCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References