Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4172614..4173233 Replicon chromosome
Accession NZ_CP089021
Organism Klebsiella pneumoniae strain 53370

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRZ76_RS20125 Protein ID WP_002892050.1
Coordinates 4173015..4173233 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRZ76_RS20120 Protein ID WP_002892066.1
Coordinates 4172614..4172988 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ76_RS20110 (4167766) 4167766..4168959 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRZ76_RS20115 (4168982) 4168982..4172128 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRZ76_RS20120 (4172614) 4172614..4172988 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRZ76_RS20125 (4173015) 4173015..4173233 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRZ76_RS20130 (4173392) 4173392..4173958 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRZ76_RS20135 (4173930) 4173930..4174070 - 141 WP_004147370.1 hypothetical protein -
LRZ76_RS20140 (4174091) 4174091..4174561 + 471 WP_002892026.1 YlaC family protein -
LRZ76_RS20145 (4174536) 4174536..4175987 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
LRZ76_RS20150 (4176088) 4176088..4176786 + 699 WP_002892021.1 GNAT family protein -
LRZ76_RS20155 (4176783) 4176783..4176923 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRZ76_RS20160 (4176923) 4176923..4177186 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T227397 WP_002892050.1 NZ_CP089021:4173015-4173233 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T227397 NZ_CP122868:218006-218113 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT227397 WP_002892066.1 NZ_CP089021:4172614-4172988 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT227397 NZ_CP122868:c217949-217894 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References