Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2802313..2803901 Replicon chromosome
Accession NZ_CP089021
Organism Klebsiella pneumoniae strain 53370

Toxin (Protein)


Gene name hipA Uniprot ID A0A331XLA5
Locus tag LRZ76_RS13605 Protein ID WP_014907613.1
Coordinates 2802313..2803635 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag LRZ76_RS13610 Protein ID WP_002904790.1
Coordinates 2803635..2803901 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ76_RS13590 (2797369) 2797369..2799870 + 2502 WP_014907611.1 fimbria/pilus outer membrane usher protein -
LRZ76_RS13595 (2799858) 2799858..2800862 + 1005 WP_014907612.1 fimbrial protein -
LRZ76_RS13600 (2801004) 2801004..2802296 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
LRZ76_RS13605 (2802313) 2802313..2803635 - 1323 WP_014907613.1 type II toxin-antitoxin system HipA family toxin Toxin
LRZ76_RS13610 (2803635) 2803635..2803901 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
LRZ76_RS13615 (2804139) 2804139..2804465 - 327 WP_004215084.1 hydroxyisourate hydrolase -
LRZ76_RS13620 (2804462) 2804462..2804962 - 501 WP_004184022.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
LRZ76_RS13625 (2804940) 2804940..2806319 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
LRZ76_RS13630 (2806665) 2806665..2807819 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
LRZ76_RS13635 (2807800) 2807800..2808723 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49139.47 Da        Isoelectric Point: 7.5367

>T227396 WP_014907613.1 NZ_CP089021:c2803635-2802313 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEFLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T227396 NZ_CP122868:217523-217630 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT227396 WP_002904790.1 NZ_CP089021:c2803901-2803635 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT227396 NZ_CP122868:c217474-217411 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331XLA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References