Detailed information of TA system TA227384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 138828..139468
• Replicon: plasmid p53374-204.7
• Accession: NZ_CP089020
• Organism: Klebsiella pneumoniae strain 53374

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: LRZ80_RS30055
• Protein ID: WP_231828924.1
• Coordinates: 138828..139241 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRZ80_RS30060
• Protein ID: WP_004213073.1
• Coordinates: 139238..139468 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ80_RS30020 (LRZ80_30025)	134208..134483	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRZ80_RS30025 (LRZ80_30030)	134546..135037	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRZ80_RS30030 (LRZ80_30035)	135086..136006	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LRZ80_RS30035 (LRZ80_30040)	136097..136500	+	404	Protein_140	GAF domain-containing protein	-
LRZ80_RS30040 (LRZ80_30045)	137019..137654	-	636	WP_165475474.1	mucoid phenotype regulator RmpA2	-
LRZ80_RS30045 (LRZ80_30050)	138071..138375	+	305	Protein_142	transposase	-
LRZ80_RS30050 (LRZ80_30055)	138398..138649	-	252	WP_186987481.1	hypothetical protein	-
LRZ80_RS30055 (LRZ80_30060)	138828..139241	-	414	WP_231828924.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ80_RS30060 (LRZ80_30065)	139238..139468	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ80_RS30065 (LRZ80_30070)	140076..141209	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRZ80_RS30070 (LRZ80_30075)	141225..141518	+	294	WP_004213076.1	hypothetical protein	-
LRZ80_RS30075 (LRZ80_30080)	141508..141714	-	207	WP_004213077.1	hypothetical protein	-
LRZ80_RS30080 (LRZ80_30085)	142066..142356	+	291	WP_004213078.1	hypothetical protein	-
LRZ80_RS30085 (LRZ80_30090)	142346..143245	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..204725	204725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14936.38 Da
• Isoelectric Point: 7.8644

>T227384 WP_231828924.1 NZ_CP089020:c139241-138828 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWV

Nucleotide

• Length: 414 bp

>T227384 NZ_CP122859:3470232-3470450 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227384 WP_004213073.1 NZ_CP089020:c139468-139238 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227384 NZ_CP122859:3469832-3470206 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: Q6U620