Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 47032..47768 Replicon plasmid p53374-204.7
Accession NZ_CP089020
Organism Klebsiella pneumoniae strain 53374

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LRZ80_RS29560 Protein ID WP_004098919.1
Coordinates 47286..47768 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LRZ80_RS29555 Protein ID WP_004213599.1
Coordinates 47032..47298 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ80_RS29530 (LRZ80_29535) 42264..42821 + 558 WP_004213590.1 recombinase family protein -
LRZ80_RS29535 (LRZ80_29540) 42824..45793 + 2970 WP_094936121.1 Tn3 family transposase -
LRZ80_RS29540 (LRZ80_29545) 45835..46329 + 495 WP_004213594.1 hypothetical protein -
LRZ80_RS29545 (LRZ80_29550) 46390..46593 + 204 WP_004213596.1 HHA domain-containing protein -
LRZ80_RS29550 (LRZ80_29555) 46607..46837 + 231 WP_004213598.1 hypothetical protein -
LRZ80_RS29555 (LRZ80_29560) 47032..47298 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LRZ80_RS29560 (LRZ80_29565) 47286..47768 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LRZ80_RS29565 (LRZ80_29570) 47969..49372 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LRZ80_RS29570 (LRZ80_29575) 49401..50033 - 633 WP_001567369.1 hypothetical protein -
LRZ80_RS29575 (LRZ80_29580) 50472..51699 + 1228 Protein_47 IS3 family transposase -
LRZ80_RS29580 (LRZ80_29585) 51695..52090 - 396 Protein_48 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla 1..204725 204725
- inside IScluster/Tn - - 42264..51699 9435


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T227382 WP_004098919.1 NZ_CP089020:47286-47768 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T227382 NZ_CP122859:2663456-2663563 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT227382 WP_004213599.1 NZ_CP089020:47032-47298 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227382 NZ_CP122859:c2663409-2663343 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References