Detailed information of TA system TA227380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5808..6333
• Replicon: plasmid p53374-100.0
• Accession: NZ_CP089017
• Organism: Klebsiella pneumoniae strain 53374

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: LRZ80_RS27115
• Protein ID: WP_013023785.1
• Coordinates: 5808..6113 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: LRZ80_RS27120
• Protein ID: WP_001568025.1
• Coordinates: 6115..6333 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ80_RS27085 (LRZ80_27090)	1519..2145	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
LRZ80_RS27090 (LRZ80_27095)	2142..2444	+	303	WP_004197636.1	hypothetical protein	-
LRZ80_RS27095 (LRZ80_27100)	2884..3678	-	795	WP_004197635.1	site-specific integrase	-
LRZ80_RS27100 (LRZ80_27105)	3876..4892	-	1017	WP_017899884.1	hypothetical protein	-
LRZ80_RS27105 (LRZ80_27110)	4903..5217	-	315	WP_053389906.1	hypothetical protein	-
LRZ80_RS27110 (LRZ80_27115)	5244..5639	-	396	WP_017899885.1	hypothetical protein	-
LRZ80_RS27115 (LRZ80_27120)	5808..6113	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
LRZ80_RS27120 (LRZ80_27125)	6115..6333	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LRZ80_RS27125 (LRZ80_27130)	6887..7576	+	690	WP_004193995.1	hypothetical protein	-
LRZ80_RS27130 (LRZ80_27135)	7608..8297	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
LRZ80_RS27135 (LRZ80_27140)	8734..8982	+	249	WP_000272716.1	hypothetical protein	-
LRZ80_RS27140 (LRZ80_27145)	8979..9551	+	573	WP_020805590.1	hypothetical protein	-
LRZ80_RS27145 (LRZ80_27150)	9582..10076	+	495	WP_020805591.1	hypothetical protein	-
LRZ80_RS27150 (LRZ80_27155)	10120..10488	+	369	WP_004197807.1	hypothetical protein	-
LRZ80_RS27155 (LRZ80_27160)	10522..10725	+	204	WP_004197808.1	HHA domain-containing protein	-
LRZ80_RS27160 (LRZ80_27165)	10739..10942	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaDHA-1 / qnrB4	-	1..100026	100026

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T227380 WP_013023785.1 NZ_CP089017:c6113-5808 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T227380 NZ_CP089017:c6113-5808 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT227380 WP_001568025.1 NZ_CP089017:c6333-6115 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT227380 NZ_CP089017:c6333-6115 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6

Antitoxin

• AlphaFold DB: A0A2J4ZZP8