Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4697550..4698047 Replicon chromosome
Accession NC_010498
Organism Escherichia coli SMS-3-5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECSMS35_RS23105 Protein ID WP_001173343.1
Coordinates 4697874..4698047 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECSMS35_RS23100 Protein ID WP_000398619.1
Coordinates 4697550..4697846 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSMS35_RS23080 4694279..4694998 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECSMS35_RS23085 4694995..4696626 - 1632 WP_001216471.1 sensor histidine kinase -
ECSMS35_RS23090 4696807..4697037 + 231 WP_000371713.1 (4Fe-4S)-binding protein -
ECSMS35_RS23095 4697049..4697321 + 273 WP_000405647.1 N-acetyltransferase -
ECSMS35_RS23100 4697550..4697846 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECSMS35_RS23105 4697874..4698047 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECSMS35_RS23110 4698166..4699683 - 1518 WP_000003799.1 lysine--tRNA ligase -
ECSMS35_RS23115 4699920..4701377 - 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T22738 WP_001173343.1 NC_010498:4697874-4698047 [Escherichia coli SMS-3-5]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T22738 NC_010498:4697874-4698047 [Escherichia coli SMS-3-5]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT22738 WP_000398619.1 NC_010498:4697550-4697846 [Escherichia coli SMS-3-5]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT22738 NC_010498:4697550-4697846 [Escherichia coli SMS-3-5]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References