Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4722935..4723745 Replicon chromosome
Accession NZ_CP089014
Organism Klebsiella pneumoniae strain 53374

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag LRZ80_RS23430 Protein ID WP_002887280.1
Coordinates 4722935..4723468 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LRZ80_RS23435 Protein ID WP_002887278.1
Coordinates 4723479..4723745 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ80_RS23425 (LRZ80_23425) 4721766..4722887 + 1122 WP_002887282.1 cupin domain-containing protein -
LRZ80_RS23430 (LRZ80_23430) 4722935..4723468 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
LRZ80_RS23435 (LRZ80_23435) 4723479..4723745 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LRZ80_RS23440 (LRZ80_23440) 4723848..4725281 - 1434 WP_231828888.1 Cu(+)/Ag(+) sensor histidine kinase -
LRZ80_RS23445 (LRZ80_23445) 4725271..4725954 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LRZ80_RS23450 (LRZ80_23450) 4726126..4727511 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
LRZ80_RS23455 (LRZ80_23455) 4727529..4727873 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T227376 WP_002887280.1 NZ_CP089014:c4723468-4722935 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T227376 NZ_CP122859:1806046-1806297 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT227376 WP_002887278.1 NZ_CP089014:c4723745-4723479 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT227376 NZ_CP122859:1806294-1806548 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References