Detailed information of TA system TA227361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 185121..185872
• Replicon: plasmid p56575-208.5
• Accession: NZ_CP089013
• Organism: Klebsiella pneumoniae strain 56575

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LRZ79_RS25975
• Protein ID: WP_048333033.1
• Coordinates: 185121..185603 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: LRZ79_RS25980
• Protein ID: WP_004902250.1
• Coordinates: 185594..185872 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ79_RS25950 (LRZ79_25950)	180942..181388	+	447	Protein_194	kinase	-
LRZ79_RS25955 (LRZ79_25955)	181520..182167	-	648	WP_014386537.1	EcsC family protein	-
LRZ79_RS25960 (LRZ79_25960)	182194..182949	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
LRZ79_RS25965 (LRZ79_25965)	183050..183442	-	393	WP_032442757.1	hypothetical protein	-
LRZ79_RS25970 (LRZ79_25970)	183547..184086	-	540	WP_004902239.1	hypothetical protein	-
LRZ79_RS25975 (LRZ79_25975)	185121..185603	-	483	WP_048333033.1	GNAT family N-acetyltransferase	Toxin
LRZ79_RS25980 (LRZ79_25980)	185594..185872	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
LRZ79_RS25985 (LRZ79_25985)	185994..186206	-	213	WP_016946198.1	hypothetical protein	-
LRZ79_RS25990 (LRZ79_25990)	186314..186655	-	342	WP_032437747.1	hypothetical protein	-
LRZ79_RS25995 (LRZ79_25995)	187561..187875	+	315	WP_023288299.1	hypothetical protein	-
LRZ79_RS26000 (LRZ79_26000)	188119..188601	-	483	WP_004210306.1	hypothetical protein	-
LRZ79_RS26005 (LRZ79_26005)	189819..190703	+	885	WP_004186900.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA	1..208529	208529
	-	flank	IS/Tn	-	-	179917..180885	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17708.53 Da
• Isoelectric Point: 8.9130

>T227361 WP_048333033.1 NZ_CP089013:c185603-185121 [Klebsiella pneumoniae]
MGMRAPESLMSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T227361 NZ_CP122844:c4229478-4229305 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT227361 WP_004902250.1 NZ_CP089013:c185872-185594 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT227361 NZ_CP122844:c4229802-4229506 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3