227359

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 100305..101041 Replicon plasmid p56575-208.5
Accession NZ_CP089013
Organism Klebsiella pneumoniae strain 56575

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag LRZ79_RS25495 Protein ID WP_004098919.1
Coordinates 100559..101041 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag LRZ79_RS25490 Protein ID WP_004213599.1
Coordinates 100305..100571 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ79_RS25465 (LRZ79_25465) 95538..96095 + 558 WP_004213590.1 recombinase family protein -
LRZ79_RS25470 (LRZ79_25470) 96098..99067 + 2970 WP_004213592.1 Tn3 family transposase -
LRZ79_RS25475 (LRZ79_25475) 99109..99603 + 495 WP_004213594.1 hypothetical protein -
LRZ79_RS25480 (LRZ79_25480) 99664..99867 + 204 WP_004213596.1 HHA domain-containing protein -
LRZ79_RS25485 (LRZ79_25485) 99881..100111 + 231 WP_004213598.1 hypothetical protein -
LRZ79_RS25490 (LRZ79_25490) 100305..100571 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
LRZ79_RS25495 (LRZ79_25495) 100559..101041 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
LRZ79_RS25500 (LRZ79_25500) 101462..102689 + 1228 Protein_104 IS3 family transposase -
LRZ79_RS25505 (LRZ79_25505) 102685..103080 - 396 Protein_105 IS3 family transposase -
LRZ79_RS25510 (LRZ79_25510) 103255..104277 - 1023 WP_004214536.1 porphobilinogen synthase -
LRZ79_RS25515 (LRZ79_25515) 104341..105687 - 1347 WP_048333011.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA 1..208529 208529
- inside IScluster/Tn - - 90348..102689 12341


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T227359 WP_004098919.1 NZ_CP089013:100559-101041 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T227359 NZ_CP122844:c4011420-4011133 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT227359 WP_004213599.1 NZ_CP089013:100305-100571 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT227359 NZ_CP122844:c4011146-4010844 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References