227357

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5062076..5062701 Replicon chromosome
Accession NZ_CP089012
Organism Klebsiella pneumoniae strain 56575

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRZ79_RS24625 Protein ID WP_002882817.1
Coordinates 5062076..5062459 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRZ79_RS24630 Protein ID WP_004150355.1
Coordinates 5062459..5062701 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRZ79_RS24610 (5059442) 5059442..5060344 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRZ79_RS24615 (5060341) 5060341..5060976 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRZ79_RS24620 (5060973) 5060973..5061902 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRZ79_RS24625 (5062076) 5062076..5062459 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRZ79_RS24630 (5062459) 5062459..5062701 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRZ79_RS24635 (5062906) 5062906..5063823 + 918 WP_004213381.1 alpha/beta hydrolase -
LRZ79_RS24640 (5063838) 5063838..5064779 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRZ79_RS24645 (5064824) 5064824..5065261 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRZ79_RS24650 (5065258) 5065258..5066118 - 861 WP_004146232.1 virulence factor BrkB family protein -
LRZ79_RS24655 (5066112) 5066112..5066711 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T227357 WP_002882817.1 NZ_CP089012:c5062459-5062076 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T227357 NZ_CP122844:3467229-3467447 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT227357 WP_004150355.1 NZ_CP089012:c5062701-5062459 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT227357 NZ_CP122844:3466829-3467203 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References