Detailed information of TA system TA227354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4522164..4523501
• Replicon: chromosome
• Accession: NZ_CP089012
• Organism: Klebsiella pneumoniae strain 56575

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: LRZ79_RS22095
• Protein ID: WP_032424556.1
• Coordinates: 4522566..4523501 (+)
• Length: 312 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: LRZ79_RS22090
• Protein ID: WP_023302769.1
• Coordinates: 4522164..4522559 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ79_RS22060 (4517408)	4517408..4517698	+	291	WP_022615704.1	hypothetical protein	-
LRZ79_RS22065 (4517763)	4517763..4518668	+	906	WP_022615703.1	zincin-like metallopeptidase domain-containing protein	-
LRZ79_RS22070 (4518861)	4518861..4519232	+	372	WP_022615702.1	hypothetical protein	-
LRZ79_RS22075 (4519406)	4519406..4521001	+	1596	WP_023302771.1	MobH family relaxase	-
LRZ79_RS22080 (4521173)	4521173..4521637	+	465	WP_022615700.1	GNAT family N-acetyltransferase	-
LRZ79_RS22085 (4521643)	4521643..4521909	-	267	WP_023302770.1	hypothetical protein	-
LRZ79_RS22090 (4522164)	4522164..4522559	+	396	WP_023302769.1	DUF6088 family protein	Antitoxin
LRZ79_RS22095 (4522566)	4522566..4523501	+	936	WP_032424556.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LRZ79_RS22100 (4523509)	4523509..4524198	-	690	WP_023302767.1	abortive infection system antitoxin AbiGi family protein	-
LRZ79_RS22105 (4524428)	4524428..4525120	-	693	WP_223272586.1	RES family NAD+ phosphorylase	-
LRZ79_RS22110 (4525641)	4525641..4527320	-	1680	WP_004212561.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (34-298)

Antitoxin

• Protein of unknown function DUF6088 (7-109)

Sequences

Toxin

Protein

• Length: 312 a.a.
• Molecular weight: 35389.66 Da
• Isoelectric Point: 5.6165

>T227354 WP_032424556.1 NZ_CP089012:4522566-4523501 [Klebsiella pneumoniae]
MLFSDVTDALGIESGAIVEKDHYIVELLHLLKPLTFDTHQLLFAGGTALAKSGIVLNRMSEDVDIKLVPTDTFLSLSRTQ
RKGIRKEIVQTISHAITATDMFSFDDAHPKVTRDEYRYNDIPVRYPQRVAQAPCLRPFIQLELMEANLLEAPEFRDIHTL
VTTLTGKAPVISAFPCATIISTQAEKLVAMLRRTAAVARNIERQDDASLVRHIYDTYCIVQAGNVDMCLLTDFFKQTIEQ
DIQRYGNQYPQFCVSPNDELKMGLDELEHNPLYKTRYQQFVAPMVYGARKVSWDEAYASFRQMALIILDTY

Nucleotide

• Length: 936 bp

>T227354 NZ_CP122844:c2485029-2484853 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14800.08 Da
• Isoelectric Point: 11.1078

>AT227354 WP_023302769.1 NZ_CP089012:4522164-4522559 [Klebsiella pneumoniae]
MTIKTRIQSRLKRSKRYVFTRDDFKDIAGYDQIGRVLRDLLKEGQLLKVGYGVYTKARRNSITGKVMPAAPRGSSAVIVE
TLERLKVRYRYEGATQTYNSGESTQIPVSPEIKTSPRFKRALSVGNSKLNG

Nucleotide

• Length: 396 bp

>AT227354 NZ_CP122844:c2484807-2484391 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA