Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 3285731..3286529 | Replicon | chromosome |
| Accession | NC_010498 | ||
| Organism | Escherichia coli SMS-3-5 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | U9XMP3 |
| Locus tag | ECSMS35_RS16235 | Protein ID | WP_000854735.1 |
| Coordinates | 3286152..3286529 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A067H947 |
| Locus tag | ECSMS35_RS16230 | Protein ID | WP_001285415.1 |
| Coordinates | 3285731..3286105 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECSMS35_RS16195 | 3281662..3282342 | + | 681 | WP_001282919.1 | WYL domain-containing protein | - |
| ECSMS35_RS16200 | 3282490..3283167 | + | 678 | WP_001097302.1 | hypothetical protein | - |
| ECSMS35_RS16205 | 3283173..3283406 | + | 234 | WP_001278287.1 | DUF905 family protein | - |
| ECSMS35_RS16210 | 3283496..3284314 | + | 819 | WP_001175148.1 | DUF945 domain-containing protein | - |
| ECSMS35_RS16215 | 3284396..3284875 | + | 480 | WP_000860076.1 | antirestriction protein | - |
| ECSMS35_RS16220 | 3284891..3285367 | + | 477 | WP_001424026.1 | RadC family protein | - |
| ECSMS35_RS16225 | 3285430..3285651 | + | 222 | WP_000692309.1 | DUF987 domain-containing protein | - |
| ECSMS35_RS16230 | 3285731..3286105 | + | 375 | WP_001285415.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| ECSMS35_RS16235 | 3286152..3286529 | + | 378 | WP_000854735.1 | TA system toxin CbtA family protein | Toxin |
| ECSMS35_RS16240 | 3286526..3287014 | + | 489 | WP_000761676.1 | hypothetical protein | - |
| ECSMS35_RS16245 | 3287026..3287223 | + | 198 | WP_000839291.1 | DUF957 domain-containing protein | - |
| ECSMS35_RS16250 | 3287308..3288174 | + | 867 | WP_001280433.1 | DUF4942 domain-containing protein | - |
| ECSMS35_RS16255 | 3288246..3288509 | + | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| ECSMS35_RS16260 | 3288506..3288832 | + | 327 | WP_000779482.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| ECSMS35_RS16265 | 3289308..3289478 | + | 171 | Protein_3069 | IS110 family transposase | - |
| ECSMS35_RS16270 | 3290289..3291272 | + | 984 | WP_001298261.1 | KpsF/GutQ family sugar-phosphate isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | kpsF / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT | 3270859..3306993 | 36134 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14061.07 Da Isoelectric Point: 8.2904
>T22735 WP_000854735.1 NC_010498:3286152-3286529 [Escherichia coli SMS-3-5]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T22735 NC_010498:3286152-3286529 [Escherichia coli SMS-3-5]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13663.32 Da Isoelectric Point: 5.8640
>AT22735 WP_001285415.1 NC_010498:3285731-3286105 [Escherichia coli SMS-3-5]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS
Download Length: 375 bp
>AT22735 NC_010498:3285731-3286105 [Escherichia coli SMS-3-5]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | U9XMP3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A067H947 |