Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3119216..3119870 Replicon chromosome
Accession NC_010498
Organism Escherichia coli SMS-3-5

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag ECSMS35_RS15285 Protein ID WP_000244781.1
Coordinates 3119216..3119623 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECSMS35_RS15290 Protein ID WP_000354046.1
Coordinates 3119604..3119870 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECSMS35_RS15265 3115173..3116906 - 1734 WP_000813176.1 single-stranded-DNA-specific exonuclease RecJ -
ECSMS35_RS15270 3116912..3117622 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECSMS35_RS15275 3117647..3118543 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
ECSMS35_RS15280 3118655..3119176 + 522 WP_001055872.1 flavodoxin FldB -
ECSMS35_RS15285 3119216..3119623 - 408 WP_000244781.1 protein YgfX Toxin
ECSMS35_RS15290 3119604..3119870 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECSMS35_RS15295 3120113..3121093 + 981 WP_000886075.1 tRNA-modifying protein YgfZ -
ECSMS35_RS15300 3121170..3121829 - 660 WP_000250269.1 hemolysin III family protein -
ECSMS35_RS15305 3121993..3122304 - 312 WP_001182958.1 N(4)-acetylcytidine aminohydrolase -
ECSMS35_RS15310 3122349..3123782 + 1434 WP_024210543.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T22734 WP_000244781.1 NC_010498:c3119623-3119216 [Escherichia coli SMS-3-5]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T22734 NC_010498:c3119623-3119216 [Escherichia coli SMS-3-5]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCTTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT22734 WP_000354046.1 NC_010498:c3119870-3119604 [Escherichia coli SMS-3-5]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT22734 NC_010498:c3119870-3119604 [Escherichia coli SMS-3-5]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References