Detailed information of TA system TA227339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 229883..230553
• Replicon: plasmid p56661-276.1
• Accession: NZ_CP089011
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LRZ77_RS29495
• Protein ID: WP_004213072.1
• Coordinates: 229883..230326 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LRZ77_RS29500
• Protein ID: WP_004213073.1
• Coordinates: 230323..230553 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS29460 (LRZ77_29455)	225264..225539	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
LRZ77_RS29465 (LRZ77_29460)	225602..226093	+	492	WP_011251327.1	DM13 domain-containing protein	-
LRZ77_RS29470 (LRZ77_29465)	226142..227062	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
LRZ77_RS29475 (LRZ77_29470)	227153..227556	+	404	Protein_252	GAF domain-containing protein	-
LRZ77_RS29480 (LRZ77_29475)	228074..228739	-	666	Protein_253	mucoid phenotype regulator RmpA2	-
LRZ77_RS29485 (LRZ77_29480)	229156..229460	+	305	Protein_254	transposase	-
LRZ77_RS29490 (LRZ77_29485)	229483..229734	-	252	WP_186987481.1	hypothetical protein	-
LRZ77_RS29495 (LRZ77_29490)	229883..230326	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ77_RS29500 (LRZ77_29495)	230323..230553	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ77_RS29505 (LRZ77_29500)	231161..232294	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LRZ77_RS29510 (LRZ77_29505)	232310..232603	+	294	WP_004213076.1	hypothetical protein	-
LRZ77_RS29515 (LRZ77_29510)	232593..232799	-	207	WP_004213077.1	hypothetical protein	-
LRZ77_RS29520 (LRZ77_29515)	233296..234276	-	981	WP_000019402.1	IS5-like element IS5 family transposase	-
LRZ77_RS29525 (LRZ77_29520)	234275..234640	+	366	WP_227545083.1	hypothetical protein	-
LRZ77_RS29530 (LRZ77_29525)	234630..235529	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2	1..276100	276100
	-	flank	IS/Tn	-	-	233296..234276	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T227339 WP_004213072.1 NZ_CP089011:c230326-229883 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T227339 NZ_CP122842:88327-88608 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT227339 WP_004213073.1 NZ_CP089011:c230553-230323 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227339 NZ_CP122842:88086-88337 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620