Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 229883..230553 | Replicon | plasmid p56661-276.1 |
| Accession | NZ_CP089011 | ||
| Organism | Klebsiella pneumoniae strain 56661 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q6U619 |
| Locus tag | LRZ77_RS29495 | Protein ID | WP_004213072.1 |
| Coordinates | 229883..230326 (-) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q6U620 |
| Locus tag | LRZ77_RS29500 | Protein ID | WP_004213073.1 |
| Coordinates | 230323..230553 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LRZ77_RS29460 (LRZ77_29455) | 225264..225539 | + | 276 | WP_004213932.1 | carboxymuconolactone decarboxylase family protein | - |
| LRZ77_RS29465 (LRZ77_29460) | 225602..226093 | + | 492 | WP_011251327.1 | DM13 domain-containing protein | - |
| LRZ77_RS29470 (LRZ77_29465) | 226142..227062 | + | 921 | WP_004213934.1 | DUF1471 domain-containing protein | - |
| LRZ77_RS29475 (LRZ77_29470) | 227153..227556 | + | 404 | Protein_252 | GAF domain-containing protein | - |
| LRZ77_RS29480 (LRZ77_29475) | 228074..228739 | - | 666 | Protein_253 | mucoid phenotype regulator RmpA2 | - |
| LRZ77_RS29485 (LRZ77_29480) | 229156..229460 | + | 305 | Protein_254 | transposase | - |
| LRZ77_RS29490 (LRZ77_29485) | 229483..229734 | - | 252 | WP_186987481.1 | hypothetical protein | - |
| LRZ77_RS29495 (LRZ77_29490) | 229883..230326 | - | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LRZ77_RS29500 (LRZ77_29495) | 230323..230553 | - | 231 | WP_004213073.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LRZ77_RS29505 (LRZ77_29500) | 231161..232294 | + | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| LRZ77_RS29510 (LRZ77_29505) | 232310..232603 | + | 294 | WP_004213076.1 | hypothetical protein | - |
| LRZ77_RS29515 (LRZ77_29510) | 232593..232799 | - | 207 | WP_004213077.1 | hypothetical protein | - |
| LRZ77_RS29520 (LRZ77_29515) | 233296..234276 | - | 981 | WP_000019402.1 | IS5-like element IS5 family transposase | - |
| LRZ77_RS29525 (LRZ77_29520) | 234275..234640 | + | 366 | WP_227545083.1 | hypothetical protein | - |
| LRZ77_RS29530 (LRZ77_29525) | 234630..235529 | + | 900 | WP_004225022.1 | nucleotide-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..276100 | 276100 | |
| - | flank | IS/Tn | - | - | 233296..234276 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 16006.74 Da Isoelectric Point: 9.3642
>T227339 WP_004213072.1 NZ_CP089011:c230326-229883 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
Download Length: 444 bp
>T227339 NZ_CP122842:88327-88608 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAGATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.73 Da Isoelectric Point: 4.4563
>AT227339 WP_004213073.1 NZ_CP089011:c230553-230323 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT227339 NZ_CP122842:88086-88337 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|