Detailed information of TA system TA227338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 126855..127765
• Replicon: plasmid p56661-276.1
• Accession: NZ_CP089011
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LRZ77_RS28955
• Protein ID: WP_004026354.1
• Coordinates: 127295..127765 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LRZ77_RS28950
• Protein ID: WP_004026357.1
• Coordinates: 126855..127298 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS28925 (LRZ77_28920)	121944..122975	+	1032	WP_004883278.1	hypothetical protein	-
LRZ77_RS28930 (LRZ77_28925)	123921..124136	-	216	Protein_142	IS200/IS605 family transposase	-
LRZ77_RS28935 (LRZ77_28930)	124138..124707	+	570	Protein_143	transposase	-
LRZ77_RS28940 (LRZ77_28935)	125062..125484	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
LRZ77_RS28945 (LRZ77_28940)	125484..126755	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
LRZ77_RS28950 (LRZ77_28945)	126855..127298	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LRZ77_RS28955 (LRZ77_28950)	127295..127765	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LRZ77_RS28960 (LRZ77_28955)	127874..128134	-	261	WP_004026352.1	hypothetical protein	-
LRZ77_RS28965 (LRZ77_28960)	128275..128972	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
LRZ77_RS28970 (LRZ77_28965)	128993..130015	+	1023	WP_231821799.1	hypothetical protein	-
LRZ77_RS28975 (LRZ77_28970)	130171..130518	+	348	WP_004883396.1	hypothetical protein	-
LRZ77_RS28980 (LRZ77_28975)	131450..131728	+	279	WP_065366041.1	hypothetical protein	-
LRZ77_RS28985 (LRZ77_28980)	131782..132555	-	774	WP_004181907.1	nuclease-related domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2	1..276100	276100
	-	flank	IS/Tn	-	-	124354..124707	353

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T227338 WP_004026354.1 NZ_CP089011:127295-127765 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T227338 NZ_CP122842:61954-62229 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT227338 WP_004026357.1 NZ_CP089011:126855-127298 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT227338 NZ_CP122842:61670-61954 [Escherichia coli]
ATACAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAAGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGTCTCAGTACTGACAATCCGTTACCGGTGCCCCGCTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9