Detailed information of TA system TA227334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 6463..6988
• Replicon: plasmid p56661-80.1
• Accession: NZ_CP089009
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: LRZ77_RS27210
• Protein ID: WP_139934942.1
• Coordinates: 6683..6988 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: LRZ77_RS27205
• Protein ID: WP_004197642.1
• Coordinates: 6463..6681 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS27155 (LRZ77_27155)	1897..2568	+	672	Protein_1	SOS response-associated peptidase	-
LRZ77_RS27160 (LRZ77_27160)	2757..3080	+	324	WP_046664160.1	hypothetical protein	-
LRZ77_RS27165 (LRZ77_27165)	3263..3517	-	255	WP_023320125.1	hypothetical protein	-
LRZ77_RS27170 (LRZ77_27170)	3634..3864	-	231	WP_049118762.1	hypothetical protein	-
LRZ77_RS27175 (LRZ77_27175)	3878..4081	-	204	WP_004213596.1	HHA domain-containing protein	-
LRZ77_RS27180 (LRZ77_27180)	4115..4483	-	369	WP_231821775.1	hypothetical protein	-
LRZ77_RS27185 (LRZ77_27185)	4527..5021	-	495	WP_114499705.1	DNA-binding protein	-
LRZ77_RS27190 (LRZ77_27190)	5051..5623	-	573	WP_231821776.1	hypothetical protein	-
LRZ77_RS27195 (LRZ77_27195)	5620..5868	-	249	WP_000272716.1	hypothetical protein	-
LRZ77_RS27200 (LRZ77_27200)	6034..6254	+	221	Protein_10	hypothetical protein	-
LRZ77_RS27205 (LRZ77_27205)	6463..6681	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
LRZ77_RS27210 (LRZ77_27210)	6683..6988	+	306	WP_139934942.1	type II toxin-antitoxin system toxin CcdB	Toxin
LRZ77_RS27215 (LRZ77_27215)	7157..7558	+	402	WP_231821777.1	hypothetical protein	-
LRZ77_RS27220 (LRZ77_27220)	7585..7899	+	315	WP_231821778.1	hypothetical protein	-
LRZ77_RS27225 (LRZ77_27225)	7914..8567	+	654	WP_231821779.1	hypothetical protein	-
LRZ77_RS27230 (LRZ77_27230)	8635..9426	+	792	WP_231821780.1	site-specific integrase	-
LRZ77_RS27235 (LRZ77_27235)	9874..10176	-	303	WP_004197636.1	hypothetical protein	-
LRZ77_RS27240 (LRZ77_27240)	10173..10799	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..80050	80050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11599.30 Da
• Isoelectric Point: 6.4672

>T227334 WP_139934942.1 NZ_CP089009:6683-6988 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T227334 NZ_CP122837:c4451248-4451075 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT227334 WP_004197642.1 NZ_CP089009:6463-6681 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT227334 NZ_CP122837:c4451572-4451276 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG