Detailed information of TA system TA227333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 15401..16044
• Replicon: plasmid p56661-46.1
• Accession: NZ_CP089007
• Organism: Klebsiella pneumoniae strain 56661

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: R9WRW3
• Locus tag: LRZ77_RS26570
• Protein ID: WP_015063455.1
• Coordinates: 15628..16044 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LRZ77_RS26565
• Protein ID: WP_001261276.1
• Coordinates: 15401..15631 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRZ77_RS26530 (LRZ77_26530)	10515..10784	+	270	WP_000339857.1	hypothetical protein	-
LRZ77_RS26535 (LRZ77_26535)	10961..11827	-	867	WP_004118283.1	replication initiation protein	-
LRZ77_RS26540 (LRZ77_26540)	12362..12466	+	105	WP_032409716.1	hypothetical protein	-
LRZ77_RS26545 (LRZ77_26545)	12595..12852	+	258	WP_000764642.1	hypothetical protein	-
LRZ77_RS26550 (LRZ77_26550)	12910..13686	-	777	WP_000015958.1	site-specific integrase	-
LRZ77_RS26555 (LRZ77_26555)	13683..14426	-	744	WP_000129823.1	hypothetical protein	-
LRZ77_RS26560 (LRZ77_26560)	14477..14827	-	351	WP_000493378.1	hypothetical protein	-
LRZ77_RS26565 (LRZ77_26565)	15401..15631	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LRZ77_RS26570 (LRZ77_26570)	15628..16044	+	417	WP_015063455.1	type II toxin-antitoxin system VapC family toxin	Toxin
LRZ77_RS26575 (LRZ77_26575)	16288..17144	+	857	Protein_18	IS3-like element ISEc15 family transposase	-
LRZ77_RS26580 (LRZ77_26580)	17426..18502	+	1077	WP_000227969.1	IS110 family transposase	-
LRZ77_RS26585 (LRZ77_26585)	18519..18797	+	279	Protein_20	IS3 family transposase	-
LRZ77_RS26590 (LRZ77_26590)	19100..20656	-	1557	WP_053897648.1	PAS domain-containing methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	armA / sul1 / qacE / aadA2 / dfrA12 / aph(6)-Id / aph(3'')-Ib	-	1..46148	46148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15036.50 Da
• Isoelectric Point: 8.5440

>T227333 WP_015063455.1 NZ_CP089007:15628-16044 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T227333 NZ_CP122837:3822107-3822385 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT227333 WP_001261276.1 NZ_CP089007:15401-15631 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT227333 NZ_CP122837:3821844-3822104 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: R9WRW3

Antitoxin

• AlphaFold DB: A0A387K023